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- W944969987 abstract "Publisher Summary This chapter discusses the use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis. The chapter describes methods for obtaining enhanced autoradiographic images of radioactive molecules in agarose or acrylamide gels. The methods described make use of solid state scintillation to overcome two problems. The first problem is that low-energy β particles are quenched within gels. Conversion of the emitted energy to visible light by an organic scintillator [PPO (2,5-diphenyloxazole)] infused into the gel increases penetration range and therefore increases the proportion of the emitted energy that is absorbed by the adjacent X-ray film. The second problem is that high-energy β particles pass through and beyond the film. Their excess energy can be captured and returned to the film in the form of visible light by placing a high density fluorescent intensifying screen beyond the film. The chapter focuses on isotope detection only after gel electrophoresis. The intensifying screen procedure for visualizing strong β emitters or γ emitters in gels is equally applicable to paper or thin layer chromatograms." @default.
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- W944969987 date "1980-01-01" @default.
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- W944969987 title "[45] The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis" @default.
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- W944969987 doi "https://doi.org/10.1016/s0076-6879(80)65047-2" @default.
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