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- W947363986 abstract "This chapter discusses the use of mutants of Rhodopseudomonas spheroids for preparation of bacteriochlorophyll precursors. Protoporphyrin is a precursor of hemes and chlorophyll, and most of the enzymatic steps leading to its formation are established. The enzymatic steps of the magnesium branch of the biosynthetic path are largely unknown, and the sequence is based mostly on products accumulated by mutant strains of photosynthetic bacteria and algae blocked in chlorophyll synthesis. The only reaction to be shown so far in vitro is the transmethylation of magnesium protoporphyrin. Mutant strains of Rhodopseudomonas spheroides which cannot form bacteriochlorophyll excrete magnesium-containing compounds into the medium when incubated under low aeration in media containing Tween and may be exploited as sources of possible substrates for enzymatic work. The preparation procedures of concentrates of some of these potential intermediates are described in the chapter. The magnesium-free derivatives are purified by chromatography on columns of polyethylene; however this approach has failed with preparations of the magnesium compounds because of the presence of Tween." @default.
- W947363986 created "2016-06-24" @default.
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- W947363986 date "1972-01-01" @default.
- W947363986 modified "2023-10-17" @default.
- W947363986 title "[36] Use of mutants of Rhodopseudomonas spheroides for preparation of bacteriochlorophyll precursors" @default.
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- W947363986 doi "https://doi.org/10.1016/0076-6879(72)24087-3" @default.
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