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- W947450170 abstract "Publisher Summary This chapter deals with DNA analysis in the diagnosis of hemoglobin disorders. The use of restriction enzymes has proved to be a very powerful tool both for dissecting DNA sequences and for analyzing the way in which they are organized into coding and noncoding regions on chromosomes. Because these enzymes recognize specific nucleotide sequences and cleave the DNA at these sites, the resulting fragments can be fractionated according to size on electrophoretic gels. With radioactive complementary DNA probes, it is possible to focus on specific genes, such as the globin genes. DNA restriction fragments are electrophoresed on 0.8% agarose gels in Tris-acetate buffer, using a horizontal gel apparatus. The chapter describes the preparation of globin-specific hybridization probes. Purifying a sequence complementary to one of the globin genes has posed problems in the past, but several groups have prepared chimeric plasmids in which a double-stranded DNA copy of human globin mRNA has been inserted. After the isolation of the recombinant plasmid, the specific double-stranded sequence can be enzymatically labeled in vitro by nick translation." @default.
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- W947450170 date "1981-01-01" @default.
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- W947450170 title "[49] DNA analysis in the diagnosis of hemoglobin disorders" @default.
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- W947450170 doi "https://doi.org/10.1016/0076-6879(81)76159-7" @default.
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