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- W947513818 abstract "This chapter discusses the current progress and future aspects of plant protoplast electrotransfection with viral nucleic acids, including several experimental results. Several factors—such as the biological condition of protoplast and viral nucleic acid samples, the capabilities of the apparatus, and the experimental conditions for transfection—affect the frequency of electrotransfection. The chapter describes the capabilities of the apparatus for electrotransfection. Under optimum conditions, almost all protoplasts are transfected with viral nucleic acid without remarkable protoplast damage. It seems possible that the optimum conditions for electrotransfection of one kind of plant protoplast are almost the same with any kind of viral nucleic acids isolated from virus particles. However, in conventional methods, using polycations, it is necessary to find the optimum conditions for the infection of protoplasts with different kinds of virus particles each time. Electrotransfection combined with in vitro mutagenesis is one of the most effective, simplest, and fastest methods for analyzing the relationships between the structure and the function of the genomes in the reverse genetics of plant viruses. Electrotransfection provides a simpler, more efficient, and more harmless method for the transfection of plant protoplasts with viral nucleic acids than the conventional method and it can be used for mass-scale experiments." @default.
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- W947513818 date "1989-01-01" @default.
- W947513818 modified "2023-09-24" @default.
- W947513818 title "Electrotransfection of Plant Protoplasts with Viral Nucleic Acids" @default.
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- W947513818 doi "https://doi.org/10.1016/s0065-3527(08)60839-8" @default.
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