Matches in SemOpenAlex for { <https://semopenalex.org/work/W950102919> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W950102919 endingPage "9836" @default.
- W950102919 startingPage "9832" @default.
- W950102919 abstract "BACKGROUND: Up to date, studies concerning capspase 3 inhibitor mainly focus on peptide/non-peptide compounds synthesis and detection. Few reports addressing inhibits chondrocytes apoptosis using silenced caspase 3 gene.OBJECTIVE: To inhibit apoptosis of chondrocytes by blocking the apoptotic cascade reaction, gene silencing of caspase 3, and transduction of caspase 3 siRNA into chondrocytes with lentivirus vector.DESIGN, TIME AND SETTING: A single sample observation was performed at the Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College of Jinan University from June 2008 to June 2009.MATERIALS: Chondrocytes were harvested from SD rats, and caspase 3 shRNA plasimid was constructed by our laboratory.METHODS: Rattus caspase 3 siRNA was synthesized and cloned into pSIH1-H1-copGFP plasmid. pSIH1-H1-copGFP-caspase 3 siRNA lentivirus was generated in 293TN cells by pPACKH1? Lentivector Packaging Kit and transducted into chondrocytes of rats.MAIN OUTCOME MEASURES: After the lentivirus was transducted into chondrocytes, the caspase 3 mRNA was tested by RT-PCR and the caspase 3 protein was tested by Western blot. Both the transducted cells and untransducted cells were induced apoptosis by tumor necrosis factor α (TNF-α). Cell apoptosis was assessed by flow cytometry, Annevin V/PI.RESULTS: The transduction rate of caspase 3 siRNA was about 90% by lentivirus vector. The expression of caspase 3 mRNA and caspase 3 protein in transducted chondrocytes was lower than the normal chondrocytes (P＜0.01). When the cells induced apoptosis by TNF-α, the apoptosis rate of the negative siRNA-chondrocytes was 7 times higher than that of caspase 3 siRNA-chondrocytes.CONCLUSION: The caspase 3 siRNA could inhibit caspase 3 expression and decrease drug-induced apoptosis of the chondrocytes." @default.
- W950102919 created "2016-06-24" @default.
- W950102919 creator A5013647360 @default.
- W950102919 creator A5032123717 @default.
- W950102919 creator A5082506597 @default.
- W950102919 date "2009-12-10" @default.
- W950102919 modified "2023-09-23" @default.
- W950102919 title "Caspase 3 siRNA抑制关节软骨细胞的凋亡" @default.
- W950102919 hasPublicationYear "2009" @default.
- W950102919 type Work @default.
- W950102919 sameAs 950102919 @default.
- W950102919 citedByCount "0" @default.
- W950102919 crossrefType "journal-article" @default.
- W950102919 hasAuthorship W950102919A5013647360 @default.
- W950102919 hasAuthorship W950102919A5032123717 @default.
- W950102919 hasAuthorship W950102919A5082506597 @default.
- W950102919 hasConcept C104317684 @default.
- W950102919 hasConcept C119056186 @default.
- W950102919 hasConcept C12519072 @default.
- W950102919 hasConcept C153911025 @default.
- W950102919 hasConcept C17991360 @default.
- W950102919 hasConcept C185592680 @default.
- W950102919 hasConcept C190283241 @default.
- W950102919 hasConcept C203014093 @default.
- W950102919 hasConcept C2522874641 @default.
- W950102919 hasConcept C2776415932 @default.
- W950102919 hasConcept C2779690655 @default.
- W950102919 hasConcept C2780727368 @default.
- W950102919 hasConcept C31573885 @default.
- W950102919 hasConcept C48297814 @default.
- W950102919 hasConcept C54009773 @default.
- W950102919 hasConcept C54355233 @default.
- W950102919 hasConcept C553184892 @default.
- W950102919 hasConcept C55493867 @default.
- W950102919 hasConcept C81885089 @default.
- W950102919 hasConcept C86803240 @default.
- W950102919 hasConcept C95444343 @default.
- W950102919 hasConcept C98424977 @default.
- W950102919 hasConceptScore W950102919C104317684 @default.
- W950102919 hasConceptScore W950102919C119056186 @default.
- W950102919 hasConceptScore W950102919C12519072 @default.
- W950102919 hasConceptScore W950102919C153911025 @default.
- W950102919 hasConceptScore W950102919C17991360 @default.
- W950102919 hasConceptScore W950102919C185592680 @default.
- W950102919 hasConceptScore W950102919C190283241 @default.
- W950102919 hasConceptScore W950102919C203014093 @default.
- W950102919 hasConceptScore W950102919C2522874641 @default.
- W950102919 hasConceptScore W950102919C2776415932 @default.
- W950102919 hasConceptScore W950102919C2779690655 @default.
- W950102919 hasConceptScore W950102919C2780727368 @default.
- W950102919 hasConceptScore W950102919C31573885 @default.
- W950102919 hasConceptScore W950102919C48297814 @default.
- W950102919 hasConceptScore W950102919C54009773 @default.
- W950102919 hasConceptScore W950102919C54355233 @default.
- W950102919 hasConceptScore W950102919C553184892 @default.
- W950102919 hasConceptScore W950102919C55493867 @default.
- W950102919 hasConceptScore W950102919C81885089 @default.
- W950102919 hasConceptScore W950102919C86803240 @default.
- W950102919 hasConceptScore W950102919C95444343 @default.
- W950102919 hasConceptScore W950102919C98424977 @default.
- W950102919 hasIssue "50" @default.
- W950102919 hasLocation W9501029191 @default.
- W950102919 hasOpenAccess W950102919 @default.
- W950102919 hasPrimaryLocation W9501029191 @default.
- W950102919 hasVolume "13" @default.
- W950102919 isParatext "false" @default.
- W950102919 isRetracted "false" @default.
- W950102919 magId "950102919" @default.
- W950102919 workType "article" @default.