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- W950587029 abstract "Publisher Summary The chapter discusses the column chromatography of oligonucleotides and polynucleotides. The separation of oligonucleotide and polynucleotide material on certain other adsorbents that have been introduced more recently, such as diethylaminoethyl cellulose (DEAE-cellulose) and methylated serum albumin, is described in this chapter. The former offers wide possibilities in the fractionation of oligonucleotides and the latter deserves special attention, because of its ability to separate nucleic acids of high molecular weight by column chromatography. The chromatographic separation of oligonucleotides, according to chain length, is discussed in this chapter. Many components can be isolated from an RNase digest, by chromatography on DEAE-cellulose, at a slightly alkaline pH. The chromatographic separation of the oligonucleotides on sephadex is also discussed in this chapter. Sephadex is a cross-linked dextran that, in principle, allows the separation of substances according to the molecular size. Chromatographic separation of nucleic acids, such as chromatographic separation on substituted cellulose anion exchangers and chromatographic separation of nucleic acids on methylated albumin columns, are also discusses in this chapter." @default.
- W950587029 created "2016-06-24" @default.
- W950587029 creator A5047423786 @default.
- W950587029 date "1963-01-01" @default.
- W950587029 modified "2023-09-23" @default.
- W950587029 title "Column Chromatography of Oligonucleotides and Polynucleotides" @default.
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- W950587029 doi "https://doi.org/10.1016/s0079-6603(08)60312-8" @default.
- W950587029 hasPublicationYear "1963" @default.
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