FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W9507757> ?p ?o ?g. }

• W9507757 abstract "The human oral cavity harbours a diverse consortium of microoganisms which has a complex relationship with host health and disease. Dental caries for example, have mulitispecies aetiologies and analysis of the mechanisms which underlie the condition is complicated by the large portion of the oral microbiota which remain uncharacterised. In vivo studies can be problematical in terms of reproducibility, ethics and inter-individual variation; reproducible and representative in vitro models of the human oral cavity are therefore of considerable utility. This doctoral thesis presents a series of in vitro or ex vivo investigations of oral microbial ecology. In order to reproduce inter-individual variation within oral consortia, a gingival model was further validated for generating salivary microcosms. Plaques were analysed using differential viable counting, PCR-DGGE, combined with principal components analysis (PCA) and Confocal laser scanning microscopy (CLSM). Data indicated that inter-individual variation in human salivary composition could be replicated in modelled microcosms (Chapter 3). In Chapter 4, poloxamer hydrogel constructs were validated for the growth and analyses of oral biofilms. Biofilms were analysed using differential viable counting, PCR-DGGE combined with principal components analysis (PCA), Confocal laser scanning microscopy (CLSM) and pH was measured using a microelectrode. Data indicated that poloxamer can support oral bacterial growth in both single and multi species biofilms, maintain compositional stability and reproduce inter-individual variation and acidogenesis. In Chapter 5, the taxonomic composition of saliva, supragingival plaque and degraded dentine was investigated in 30 volunteers [10 healthy and 20 Type 2 diabetes mellitus (T2DM)], using culture, PCR-DGGE and cluster analysis combined with PCA. Unique PCR amplicons were sequenced for identity. Dental caries incidence was elevated in T2DM compared to healthy individuals but the oral bacterial composition in supragingival plaque between two groups was not significantly different. Salivary eubacterial profiles from T2DM patients with and without caries clustered separately. With respect to bacterial consortia associated with degraded dentine, Prevotella nigrescens, Neisseria mucosa and Streptococcus sanguinis were associated with diabetes. In order to investigate potential familial factors in oral disease aetiology, the oral microbiotas of members of 10 families [5 caries free (CF) and 5 severe early childhood caries (SECC) families] were characterised using differential viable counting, together with PCR-DGGE, cluster analysis and PCA (Chapter 6). There was a highly significant association between numbers of lactobacilli in saliva and plaque and caries experience in SECC patients. Additionally, the numbers of streptococci in dental plaque were significantly higher in SECC patients than in CF controls. Regression analysis showed that plaque index score correlated significantly with DMFT and with lactobacillus abundance in dental plaque in a highly predictive manner. However, eubacterial fingerprints did not cluster within or between CF and SECC groups and bacterial counts for the three sampling sites in SECC did not differ significantly. Numbers of anaerobes, facultative anaerobes and streptococci of parents and children were correlated. In terms of familial microbial similarities, DGGE concordance between children, their mothers and their siblings ranged between 43 percent to 73 percent, and 35 percent to 82 percent, respectively. Data indicated a possible aetiological role of lactobacilli and potentially provide novel means of prediction." @default.
• W9507757 created "2016-06-24" @default.
• W9507757 creator A5058291514 @default.
• W9507757 date "2011-07-01" @default.
• W9507757 modified "2023-09-24" @default.
• W9507757 title "Bacteriological investigations of the human oral microbiota in health and disease" @default.
• W9507757 cites W129926023 @default.
• W9507757 cites W1485711692 @default.
• W9507757 cites W1493139552 @default.
• W9507757 cites W1512511942 @default.
• W9507757 cites W1602329895 @default.
• W9507757 cites W1604878006 @default.
• W9507757 cites W1881563271 @default.
• W9507757 cites W1940818277 @default.
• W9507757 cites W1965026294 @default.
• W9507757 cites W1967358601 @default.
• W9507757 cites W1969604041 @default.
• W9507757 cites W1970387669 @default.
• W9507757 cites W1971627353 @default.
• W9507757 cites W1980674272 @default.
• W9507757 cites W1980881947 @default.
• W9507757 cites W1983885627 @default.
• W9507757 cites W1984123458 @default.
• W9507757 cites W1989805249 @default.
• W9507757 cites W2000072693 @default.
• W9507757 cites W2003193619 @default.
• W9507757 cites W2005284647 @default.
• W9507757 cites W2005746105 @default.
• W9507757 cites W2011809650 @default.
• W9507757 cites W2014396954 @default.
• W9507757 cites W2015640111 @default.
• W9507757 cites W2016952802 @default.
• W9507757 cites W2017981544 @default.
• W9507757 cites W2019400123 @default.
• W9507757 cites W2024903291 @default.
• W9507757 cites W2027885195 @default.
• W9507757 cites W2032475200 @default.
• W9507757 cites W2039262609 @default.
• W9507757 cites W2043449808 @default.
• W9507757 cites W2047820042 @default.
• W9507757 cites W2050663558 @default.
• W9507757 cites W2054016842 @default.
• W9507757 cites W2058010940 @default.
• W9507757 cites W2058546776 @default.
• W9507757 cites W2059492480 @default.
• W9507757 cites W2060512867 @default.
• W9507757 cites W2067386614 @default.
• W9507757 cites W2068354112 @default.
• W9507757 cites W2070312868 @default.
• W9507757 cites W2070981217 @default.
• W9507757 cites W2072626216 @default.
• W9507757 cites W2073919816 @default.
• W9507757 cites W2074906205 @default.
• W9507757 cites W2083567298 @default.
• W9507757 cites W2085682566 @default.
• W9507757 cites W2093358055 @default.
• W9507757 cites W2093891969 @default.
• W9507757 cites W2094991789 @default.
• W9507757 cites W2095025839 @default.
• W9507757 cites W2095566926 @default.
• W9507757 cites W2095927934 @default.
• W9507757 cites W2096353592 @default.
• W9507757 cites W2096570604 @default.
• W9507757 cites W2100638494 @default.
• W9507757 cites W2101758948 @default.
• W9507757 cites W2102001108 @default.
• W9507757 cites W2104771379 @default.
• W9507757 cites W2105046278 @default.
• W9507757 cites W2107334033 @default.
• W9507757 cites W2107649652 @default.
• W9507757 cites W2109349485 @default.
• W9507757 cites W2112445954 @default.
• W9507757 cites W2114342603 @default.
• W9507757 cites W2114707634 @default.
• W9507757 cites W2121527198 @default.
• W9507757 cites W2126446070 @default.
• W9507757 cites W2127702166 @default.
• W9507757 cites W2138791188 @default.
• W9507757 cites W2138878714 @default.
• W9507757 cites W2139962100 @default.
• W9507757 cites W2143526883 @default.
• W9507757 cites W2144826002 @default.
• W9507757 cites W2145645349 @default.
• W9507757 cites W2147221919 @default.
• W9507757 cites W2147724119 @default.
• W9507757 cites W2148803346 @default.
• W9507757 cites W2152268880 @default.
• W9507757 cites W2158135731 @default.
• W9507757 cites W2159492867 @default.
• W9507757 cites W2161526892 @default.
• W9507757 cites W2166664905 @default.
• W9507757 cites W2167820176 @default.
• W9507757 cites W2253670786 @default.
• W9507757 cites W2290846716 @default.
• W9507757 cites W2345903479 @default.
• W9507757 cites W2415556931 @default.
• W9507757 cites W2520432709 @default.
• W9507757 cites W68978804 @default.
• W9507757 cites W83867207 @default.
• W9507757 cites W23796610 @default.

• next