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- W950805422 abstract "Publisher Summary This chapter discusses Methionine Biosynthesis (Hog Liver). This enzyme has been isolated and purified from cultures of E. coli 113-3 grown on limiting quantitites of vitamin B 12 and in the absence of methionine. Under these circumstances derepression occurs and extracts of high initial specific activity can be obtained. For this reason it is easier to isolate purified preparations of the enzyme from E. coli 113-3 than from liver. The isolation of 5-methyl tetrahydrofolate-bomocysteine transmethylase has been undertaken from pig liver. The relative improvement of the specific activity of the enzyme is approximately 250 times, but it is still far below the specific activity of the most purified enzyme preparations isolated from mutant 113-3 of E. coli grown on Vitamin B 12 and methionine. The properties of the liver enzyme resemble those of the enzyme obtained from E. coli 113-3 in that it contains a derivative of vitamin B 12 as a cofactor or prosthetic group." @default.
- W950805422 created "2016-06-24" @default.
- W950805422 creator A5076528194 @default.
- W950805422 date "1971-01-01" @default.
- W950805422 modified "2023-09-27" @default.
- W950805422 title "[187] Methionine biosynthesis (hog liver)" @default.
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- W950805422 doi "https://doi.org/10.1016/0076-6879(71)17064-4" @default.
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