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- W951431419 abstract "Publisher Summary Polyacrylamide gel electrophoresis is an excellent and widely employed method for the separation and identification of soluble enzymes, but membrane proteins, including enzymes, present difficulties because of their presumed lipoprotein character. Large lipoprotein molecules or aggregates are unlikely to enter gels composed of 5–10% acrylamide that is the best compromise between firmness and large pore size, or, if they enter, fail to resolve satisfactorily. Consequently, it is necessary to solubilize or disaggregate the membrane proteins prior to electrophoresis. Disaggregation or solubilization has most often been accomplished by the method of Takayama or modifications in which membranes are treated with phenol, acetic acid, and urea, subsequent electrophoresis being accomplished in gels containing 5 M urea and 35 % acetic acid." @default.
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- W951431419 date "1974-01-01" @default.
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- W951431419 title "[8] Solubilization and polyacrylamide gel electrophoresis of me mbrane enzymes with detergents" @default.
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- W951431419 doi "https://doi.org/10.1016/0076-6879(74)32011-3" @default.
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