FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W951561641> ?p ?o ?g. }

• W951561641 endingPage "508" @default.
• W951561641 startingPage "488" @default.
• W951561641 abstract "This chapter presents the procedure for preparation and assay of the intermediate filament proteins desrnin and vimentin. Assays involve identification of these two proteins on two-dimensional gels, production of specific antisera, and immunofluorescence. Testing the specificity of the antisera and detection of subunits in different cell types can be accomplished with the technique of immunoautoradiography. Purification of desmin from gizzard smooth muscle involves extraction of most other proteins from the tissue, followed by solubilization of desrain from the cytoskeletal residue with acetic acid. Neutralization of the acetic acid solution leads to precipitation of the desmin; this precipitate can be redissolved in acetic acid and reprecipitated. The result of this cycling procedure is a preparation consisting primarily of desmin, with minor amounts of actin, vimentin, and synemin. As with any protein enrichment procedure, care should be taken to minimize proteolysis, denaturation, and modification of the proteins. Processing is carried out at 0–4 °, and appropriate protease inhibitors are added to solutions. Tissue residues are extracted and washed by centrifugation; the volume ratio of supernatant to pellet and the frequency of washes should be maximized within the limits of convenience." @default.
• W951561641 created "2016-06-24" @default.
• W951561641 creator A5001924858 @default.
• W951561641 creator A5024797290 @default.
• W951561641 date "1982-01-01" @default.
• W951561641 modified "2023-09-25" @default.
• W951561641 title "[46] Preparation and assay of the intermediate filament proteins desmin and vimentin" @default.
• W951561641 cites W1232909914 @default.
• W951561641 cites W1503540314 @default.
• W951561641 cites W1504754828 @default.
• W951561641 cites W1606423922 @default.
• W951561641 cites W1893651774 @default.
• W951561641 cites W1969023545 @default.
• W951561641 cites W1979790421 @default.
• W951561641 cites W1980755114 @default.
• W951561641 cites W1985887257 @default.
• W951561641 cites W2004023258 @default.
• W951561641 cites W2008820456 @default.
• W951561641 cites W2011071723 @default.
• W951561641 cites W2014974032 @default.
• W951561641 cites W2026444465 @default.
• W951561641 cites W2036571146 @default.
• W951561641 cites W2053226801 @default.
• W951561641 cites W2055121968 @default.
• W951561641 cites W2057325137 @default.
• W951561641 cites W2061490756 @default.
• W951561641 cites W2063308605 @default.
• W951561641 cites W2063721147 @default.
• W951561641 cites W2066274406 @default.
• W951561641 cites W2068654541 @default.
• W951561641 cites W2083453910 @default.
• W951561641 cites W2085129302 @default.
• W951561641 cites W2100837269 @default.
• W951561641 cites W2106321492 @default.
• W951561641 cites W2152351059 @default.
• W951561641 cites W2164877738 @default.
• W951561641 cites W4313315365 @default.
• W951561641 cites W4313377000 @default.
• W951561641 doi "https://doi.org/10.1016/0076-6879(82)85048-9" @default.
• W951561641 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6750318" @default.
• W951561641 hasPublicationYear "1982" @default.
• W951561641 type Work @default.
• W951561641 sameAs 951561641 @default.
• W951561641 citedByCount "22" @default.
• W951561641 crossrefType "book-chapter" @default.
• W951561641 hasAuthorship W951561641A5001924858 @default.
• W951561641 hasAuthorship W951561641A5024797290 @default.
• W951561641 hasConcept C142669718 @default.
• W951561641 hasConcept C147447768 @default.
• W951561641 hasConcept C1491633281 @default.
• W951561641 hasConcept C153911025 @default.
• W951561641 hasConcept C159654299 @default.
• W951561641 hasConcept C181199279 @default.
• W951561641 hasConcept C185592680 @default.
• W951561641 hasConcept C203014093 @default.
• W951561641 hasConcept C204232928 @default.
• W951561641 hasConcept C2779072444 @default.
• W951561641 hasConcept C2781307694 @default.
• W951561641 hasConcept C43617362 @default.
• W951561641 hasConcept C55493867 @default.
• W951561641 hasConcept C7623868 @default.
• W951561641 hasConcept C78383274 @default.
• W951561641 hasConcept C86803240 @default.
• W951561641 hasConcept C91523082 @default.
• W951561641 hasConceptScore W951561641C142669718 @default.
• W951561641 hasConceptScore W951561641C147447768 @default.
• W951561641 hasConceptScore W951561641C1491633281 @default.
• W951561641 hasConceptScore W951561641C153911025 @default.
• W951561641 hasConceptScore W951561641C159654299 @default.
• W951561641 hasConceptScore W951561641C181199279 @default.
• W951561641 hasConceptScore W951561641C185592680 @default.
• W951561641 hasConceptScore W951561641C203014093 @default.
• W951561641 hasConceptScore W951561641C204232928 @default.
• W951561641 hasConceptScore W951561641C2779072444 @default.
• W951561641 hasConceptScore W951561641C2781307694 @default.
• W951561641 hasConceptScore W951561641C43617362 @default.
• W951561641 hasConceptScore W951561641C55493867 @default.
• W951561641 hasConceptScore W951561641C7623868 @default.
• W951561641 hasConceptScore W951561641C78383274 @default.
• W951561641 hasConceptScore W951561641C86803240 @default.
• W951561641 hasConceptScore W951561641C91523082 @default.
• W951561641 hasLocation W9515616411 @default.
• W951561641 hasLocation W9515616412 @default.
• W951561641 hasOpenAccess W951561641 @default.
• W951561641 hasPrimaryLocation W9515616411 @default.
• W951561641 hasRelatedWork W129824029 @default.
• W951561641 hasRelatedWork W1569570275 @default.
• W951561641 hasRelatedWork W1880723896 @default.
• W951561641 hasRelatedWork W1975322859 @default.
• W951561641 hasRelatedWork W2008853752 @default.
• W951561641 hasRelatedWork W2062332518 @default.
• W951561641 hasRelatedWork W2078234352 @default.
• W951561641 hasRelatedWork W2123748731 @default.
• W951561641 hasRelatedWork W2188631660 @default.
• W951561641 hasRelatedWork W4239594472 @default.
• W951561641 isParatext "false" @default.
• W951561641 isRetracted "false" @default.
• W951561641 magId "951561641" @default.

• next