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- W951806315 abstract "A new methodology for creation of nanoscale bioreactor insidethe separation capillary in capillary electrophoresis wasdeveloped. In principle, solutions of reactants are injected byrelatively low pressure as a series of consecutive narrow plugswith slightly parabolic profiles due to laminar nature of thehydrodynamic flow inside the capillary. Reaction mixture isthen mixed by combination of longitudinal and transversediffusion within minute period. This procedure provideshomogenous reaction mixture with easily changeable composition.Kinetic and inhibition studies of cytochrome P450 2C9 (CYP2C9)reaction with diclofenac as a probe substrate andsulfaphenazole as a probe inhibitor were performed in order toprove the applicability of proposed methodology for on-linescreenings of CYP2C9 inhibitors in early stages of a new drugdevelopment. As a result apparent Michaelis constant of 2.66 +-0.18 uM, apparent maximum reaction velocity of 7.91 +- 0.22nmol/min/nmol, Hill coefficient of 1.59 +- 0.16, 50 %inhibitory concentration of 0.94 +- 0.04 uM and apparentinhibition constant of 0.39 +- 0.07 uM were determined. Allthese values are in agreement with literature data obtained bydifferent techniques. What is more, developed methodologyenables reduction of CYP2C9 solution consumption to 0.6 nl peranalysis." @default.
- W951806315 created "2016-06-24" @default.
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- W951806315 date "2012-12-02" @default.
- W951806315 modified "2023-09-27" @default.
- W951806315 title "Utilization of In-capillary Nanoreactor: Inhibition Study of Cytochrome P450 2C9 Isoform Reaction with Diclofenac and Sulfaphenazole by Means of Capillary Electrophoresis" @default.
- W951806315 hasPublicationYear "2012" @default.
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