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- W95267802 abstract "Since its emergence almost 20 years ago (Schwartz et al., Science 1995), optical mapping has undergone a transition from laboratory technique to commercially available data generation method. In line with this transition, it is only relatively recently that optical mapping data has started to be used for scaffolding contigs and assembly validation in large-scale sequencing projects — for example, the goat (Dong et al., Nature Biotech. 2013) and amborella (Chamala et al., Science 2013) genomes. One major hurdle to the wider use of optical mapping data is the efficient alignment of in silico digested contigs to an optical map. We develop Twin to tackle this very problem. Twin is the first index-based method for aligning in silico digested contigs to an optical map. Our results demonstrate that Twin is an order of magnitude faster than competing methods on the largest genome. Most importantly, it is specifically designed to be capable of dealing with large eukaryote genomes and thus is the only non-proprietary method capable of completing the alignment for the budgerigar genome in a reasonable amount of CPU time." @default.
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- W95267802 date "2014-01-01" @default.
- W95267802 modified "2023-09-26" @default.
- W95267802 title "Efficient Indexed Alignment of Contigs to Optical Maps" @default.
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- W95267802 doi "https://doi.org/10.1007/978-3-662-44753-6_6" @default.
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