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- W953043123 abstract "为提高传染性法氏囊病病毒（IBDV）多聚蛋白（VP2／4／3）DNA疫苗的免疫效果，应用重叠延伸剪切技术（splicing by overlapping extension）将IBDVVP2／4／3基因与鸡白细胞介素18（ChIL-18）基因分别经3次PCR获得融合基因片段VP2／4／3-ChIL-18和ChIL-18-VP2／4／3，将融合基因片段定向插入真核表达载体pCI中，获得重组质粒pCI-VP2／4／3-ChIL-18和pCI-ChlL-18-VP2／4／3；应用PCR法，在本实验室已成功构建的重组真核表达载体pCI—VP2／4／3和pCI—ChlL18的基础上，构建VP2／4／3基因和ChlI。18基因的共表达载体co-pCI-VP2／4／3-ChIL-18．在脂质体介导下将上述重组真核表达载体转染Vero细胞，间接免疫荧光证实重组质粒能正常表达目的蛋白，表达的蛋白具有免疫反应性．这为进一步研究ChIL-18的分子免疫佐剂作用及研制高效、价廉的IBDVDNA疫苗提供了实验依据．" @default.
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- W953043123 date "2010-01-01" @default.
- W953043123 modified "2023-10-18" @default.
- W953043123 title "IBDVVP2／4／3、ChIL-18共表达载体的构建及在Vero细胞上的表达" @default.
- W953043123 hasPublicationYear "2010" @default.
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