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• W95366919 abstract "Article1 April 1986free access Recombination in a plant virus: template-switching in cauliflower mosaic virus Nigel Grimsley Nigel Grimsley Friedrich Miescher-Institut, PO Box 2543, CH-4002 Basel, Switzerland Search for more papers by this author Thomas Hohn Thomas Hohn Friedrich Miescher-Institut, PO Box 2543, CH-4002 Basel, Switzerland Search for more papers by this author Barbara Hohn Barbara Hohn Friedrich Miescher-Institut, PO Box 2543, CH-4002 Basel, Switzerland Search for more papers by this author Nigel Grimsley Nigel Grimsley Friedrich Miescher-Institut, PO Box 2543, CH-4002 Basel, Switzerland Search for more papers by this author Thomas Hohn Thomas Hohn Friedrich Miescher-Institut, PO Box 2543, CH-4002 Basel, Switzerland Search for more papers by this author Barbara Hohn Barbara Hohn Friedrich Miescher-Institut, PO Box 2543, CH-4002 Basel, Switzerland Search for more papers by this author Author Information Nigel Grimsley1, Thomas Hohn1 and Barbara Hohn1 1Friedrich Miescher-Institut, PO Box 2543, CH-4002 Basel, Switzerland The EMBO Journal (1986)5:641-646https://doi.org/10.1002/j.1460-2075.1986.tb04261.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A hybrid plasmid, containing tandemly arranged pieces of two different but well-defined cauliflower mosaic virus (CaMV) genomes, was used to study the mechanism by which infectious viral DNA can escape from transforming DNA. Systemic viral infection followed inoculation of Brassica plants with a strain of Agrobacterium tumefaciens containing the hybrid plasmid in its T-DNA. Restriction mapping of uncloned viral DNA from these plants, and sequencing of relevant portions of cloned viral DNA, showed that the majority of viral progeny were probably descendants of DNA produced by transcription/reverse transcription of the viral genome, thus providing further evidence for the hypothesis that this process is normally involved in viral replication. The reverse transcriptase enzyme, which is thought to undergo an intramolecular template switch during viral replication, is shown to move very close to the 5′ end of the terminal repeat on the 35S RNA molecule before switching templates. The remaining minority of viral genomes can best be explained as arising from products of recombination between homologous regions of CaMV DNA. Previous ArticleNext Article Volume 5Issue 41 April 1986In this issue RelatedDetailsLoading ..." @default.
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• W95366919 title "Recombination in a plant virus: template-switching in cauliflower mosaic virus" @default.
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