Matches in SemOpenAlex for { <https://semopenalex.org/work/W95553002> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W95553002 abstract "When plasminogen is bound to cells its activation is markedly enhanced compared to the reaction in solution, a key step in regulation of thrombolysis and cell migration. To study the mechanism of cell-surface plasminogen activation we developed a monoclonal antibody (mAb 51) that reacts with cell-associated plasminogen in blood in the presence of a high molar excess of soluble plasminogen. Thus, mAb 51 detects receptor-induced binding sites (RIBS) induced in plasminogen upon its interaction with cells. The objective of our study was to define the RIBS epitope in plasminogen recognized by mAb 51. Plasminogen was denatured, reduced and alkylated and then digested with trypsin. Using western blotting and a specific Mab 51-based ELISA for plasminogen, we established the concentration of trypsin that resulted in maximal digestion of plasminogen, while still retaining reactivity with mAb 51. The plasminogen digest was applied to a mAb 51 immunoaffinity column. MALDI analysis of the eluate yielded a single peak with a mass of 3,076 m/z. In LC-MS-MS, a peak with the same mass was obtained and assigned to a peptide corresponding to K177-Y154, spanning a domain linking plasminogen kringles 1 and 2. Our data suggest that this domain becomes accessible when plasminogen interacts with cells. Thus, this conformational change detected by mAb 51 is likely to contribute to the ability of plasminogen to be activated on the cell surface." @default.
- W95553002 created "2016-06-24" @default.
- W95553002 creator A5009194991 @default.
- W95553002 creator A5014757822 @default.
- W95553002 creator A5050414017 @default.
- W95553002 date "2010-04-01" @default.
- W95553002 modified "2023-09-27" @default.
- W95553002 title "Identification of a receptor‐induced binding site (RIBS) in plasminogen induced by its interaction with cells" @default.
- W95553002 doi "https://doi.org/10.1096/fasebj.24.1_supplement.837.1" @default.
- W95553002 hasPublicationYear "2010" @default.
- W95553002 type Work @default.
- W95553002 sameAs 95553002 @default.
- W95553002 citedByCount "0" @default.
- W95553002 crossrefType "journal-article" @default.
- W95553002 hasAuthorship W95553002A5009194991 @default.
- W95553002 hasAuthorship W95553002A5014757822 @default.
- W95553002 hasAuthorship W95553002A5050414017 @default.
- W95553002 hasConcept C105702510 @default.
- W95553002 hasConcept C107824862 @default.
- W95553002 hasConcept C116834253 @default.
- W95553002 hasConcept C12554922 @default.
- W95553002 hasConcept C160306043 @default.
- W95553002 hasConcept C170493617 @default.
- W95553002 hasConcept C185592680 @default.
- W95553002 hasConcept C18903297 @default.
- W95553002 hasConcept C55493867 @default.
- W95553002 hasConcept C70721500 @default.
- W95553002 hasConcept C86803240 @default.
- W95553002 hasConcept C95444343 @default.
- W95553002 hasConceptScore W95553002C105702510 @default.
- W95553002 hasConceptScore W95553002C107824862 @default.
- W95553002 hasConceptScore W95553002C116834253 @default.
- W95553002 hasConceptScore W95553002C12554922 @default.
- W95553002 hasConceptScore W95553002C160306043 @default.
- W95553002 hasConceptScore W95553002C170493617 @default.
- W95553002 hasConceptScore W95553002C185592680 @default.
- W95553002 hasConceptScore W95553002C18903297 @default.
- W95553002 hasConceptScore W95553002C55493867 @default.
- W95553002 hasConceptScore W95553002C70721500 @default.
- W95553002 hasConceptScore W95553002C86803240 @default.
- W95553002 hasConceptScore W95553002C95444343 @default.
- W95553002 hasIssue "S1" @default.
- W95553002 hasLocation W955530021 @default.
- W95553002 hasOpenAccess W95553002 @default.
- W95553002 hasPrimaryLocation W955530021 @default.
- W95553002 hasRelatedWork W1566867371 @default.
- W95553002 hasRelatedWork W1569704448 @default.
- W95553002 hasRelatedWork W176069916 @default.
- W95553002 hasRelatedWork W1975013617 @default.
- W95553002 hasRelatedWork W1981571842 @default.
- W95553002 hasRelatedWork W1983727861 @default.
- W95553002 hasRelatedWork W1989685699 @default.
- W95553002 hasRelatedWork W2021377094 @default.
- W95553002 hasRelatedWork W2040992661 @default.
- W95553002 hasRelatedWork W2049631846 @default.
- W95553002 hasRelatedWork W2062392379 @default.
- W95553002 hasRelatedWork W2067229110 @default.
- W95553002 hasRelatedWork W2069334113 @default.
- W95553002 hasRelatedWork W2074103165 @default.
- W95553002 hasRelatedWork W2076374640 @default.
- W95553002 hasRelatedWork W2084425906 @default.
- W95553002 hasRelatedWork W2167413291 @default.
- W95553002 hasRelatedWork W2172204542 @default.
- W95553002 hasRelatedWork W2277469464 @default.
- W95553002 hasRelatedWork W2558300628 @default.
- W95553002 hasVolume "24" @default.
- W95553002 isParatext "false" @default.
- W95553002 isRetracted "false" @default.
- W95553002 magId "95553002" @default.
- W95553002 workType "article" @default.