FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W95593920> ?p ?o ?g. }

• W95593920 abstract "Anti nucleolar antibodies are estimated to be present in about 5-10% of anti-nuclear antibodies-positive (ANA), but only in about 15% of cases (sometimes in lower percentages) it is possible to assess precisely the specificity of antibodies responsible for this pattern of ANA. Nucleoli are composed of many proteins, e.g., nucleolin, fibrillarin, Pm-Scl (spliceosome component), RNA-polymerases and different sizes rRNA transcripts. Nucleoli also contain annexins (ANX) II and V. Many of these proteins are target molecules for autoantibodies generated in connective tissue diseases (CTD)--especially in scleroderma and overlap syndromes.The aim of this study was to work out methods for differentiation of antibody specificities in the case of sera ANA-positive with the nucleolar pattern in indirect immunofluorescence (IIF), with a particular concern directed to anti-Annexin V antibodies (belonging to anticofactor antibody system--characteristic of antiphospholipid syndrome [APS]).In the sera of the tested group of 12 (selected from 150 subjects suffering from different kinds of CTD) ANA-positive (Western-blott negative) and showing the nucleolar type of ANA pattern patients, the following autoantibodies were assessed: ANA-IIF, anti-Pm-Scl (overlap syndrome marker), anticardiolipin, antiannexin V, anti-RNA-ase--all using the ELISA method. With the use of the Western-blott method a basic set marker autoantibody in CTD was estimated, and the RNA-ase was used for elimination (by enzymatic digestion) of RNA-s and RNA-protein complexes--a possible target for autoantibodies giving the nucleolar pattern.In 5 out of 12 sera (41.7%) of ANA-positive/Western-blott-negative CTD patients antibodies against Annexin V, cardiolipin, Pm-Scl were detected. In 2 out of 12 patient sera antibodies to the RNA-ase were found. None of the tested sera showed the presence of autoantibodies against antigen Scl-70 (scleroderma marker), and SS-A (Sjögren syndrome marker), which can also give the atypical nucleolar ANA-pattern. Only in few sera my ositis--blotts showed antibodies to antigen Ku (nuclear protein), Jo-1 and PI-7 (anti-tRNA synthetases--polymyositis markers)--myositis and overlap syndrome markers. In 80% of tested sera the co-appearance of anti-ANX-V and anticardiolipin antibodies was observed. The presence of antibodies against ANX-V together with Pm-Scl was confirmed in 60% of sera The RNA-ase, used as a specific rRNA and RNP-protein blocker, resulted in a partial or total disappearance of the nucleolar pattern, but independently of the tested serum specificity (anti-ANX-V, anti-Pm-Scl or both together). The observed RNA-ase treatment of the Hep-2 cells effects are equivocal and they may result from either binding or digestion the antigen by the RNA-ase, but also from forming a sterical hindrance for the autoantigen binding to another nucleolar antigens.These preliminary results are interesting, especially as concerns anti-ANX-V autoantigens (associated with APS), but they require further research including bigger groups of CTD patients." @default.
• W95593920 created "2016-06-24" @default.
• W95593920 creator A5024255363 @default.
• W95593920 creator A5033091293 @default.
• W95593920 creator A5037227316 @default.
• W95593920 creator A5051755582 @default.
• W95593920 date "2008-12-01" @default.
• W95593920 modified "2023-09-25" @default.
• W95593920 title "Antinucleolar antibodies in diagnostics of antiphospholipid syndrome" @default.
• W95593920 doi "https://doi.org/10.20452/pamw.562" @default.
• W95593920 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19562966" @default.
• W95593920 hasPublicationYear "2008" @default.
• W95593920 type Work @default.
• W95593920 sameAs 95593920 @default.
• W95593920 citedByCount "3" @default.
• W95593920 countsByYear W955939202016 @default.
• W95593920 crossrefType "journal-article" @default.
• W95593920 hasAuthorship W95593920A5024255363 @default.
• W95593920 hasAuthorship W95593920A5033091293 @default.
• W95593920 hasAuthorship W95593920A5037227316 @default.
• W95593920 hasAuthorship W95593920A5051755582 @default.
• W95593920 hasConcept C101180180 @default.
• W95593920 hasConcept C104317684 @default.
• W95593920 hasConcept C149443304 @default.
• W95593920 hasConcept C153911025 @default.
• W95593920 hasConcept C159654299 @default.
• W95593920 hasConcept C163764329 @default.
• W95593920 hasConcept C183873130 @default.
• W95593920 hasConcept C190062978 @default.
• W95593920 hasConcept C203014093 @default.
• W95593920 hasConcept C2776007122 @default.
• W95593920 hasConcept C2776525860 @default.
• W95593920 hasConcept C2776874634 @default.
• W95593920 hasConcept C54355233 @default.
• W95593920 hasConcept C67705224 @default.
• W95593920 hasConcept C86803240 @default.
• W95593920 hasConceptScore W95593920C101180180 @default.
• W95593920 hasConceptScore W95593920C104317684 @default.
• W95593920 hasConceptScore W95593920C149443304 @default.
• W95593920 hasConceptScore W95593920C153911025 @default.
• W95593920 hasConceptScore W95593920C159654299 @default.
• W95593920 hasConceptScore W95593920C163764329 @default.
• W95593920 hasConceptScore W95593920C183873130 @default.
• W95593920 hasConceptScore W95593920C190062978 @default.
• W95593920 hasConceptScore W95593920C203014093 @default.
• W95593920 hasConceptScore W95593920C2776007122 @default.
• W95593920 hasConceptScore W95593920C2776525860 @default.
• W95593920 hasConceptScore W95593920C2776874634 @default.
• W95593920 hasConceptScore W95593920C54355233 @default.
• W95593920 hasConceptScore W95593920C67705224 @default.
• W95593920 hasConceptScore W95593920C86803240 @default.
• W95593920 hasLocation W955939201 @default.
• W95593920 hasLocation W955939202 @default.
• W95593920 hasOpenAccess W95593920 @default.
• W95593920 hasPrimaryLocation W955939201 @default.
• W95593920 hasRelatedWork W111243903 @default.
• W95593920 hasRelatedWork W1566600037 @default.
• W95593920 hasRelatedWork W1975168386 @default.
• W95593920 hasRelatedWork W1995452469 @default.
• W95593920 hasRelatedWork W1998591373 @default.
• W95593920 hasRelatedWork W2014223882 @default.
• W95593920 hasRelatedWork W2133518113 @default.
• W95593920 hasRelatedWork W2133854933 @default.
• W95593920 hasRelatedWork W2148407089 @default.
• W95593920 hasRelatedWork W2207826989 @default.
• W95593920 hasRelatedWork W2318194835 @default.
• W95593920 hasRelatedWork W2347799284 @default.
• W95593920 hasRelatedWork W2404873907 @default.
• W95593920 hasRelatedWork W2412876655 @default.
• W95593920 hasRelatedWork W2464508995 @default.
• W95593920 hasRelatedWork W3002961633 @default.
• W95593920 hasRelatedWork W3033126259 @default.
• W95593920 hasRelatedWork W325933835 @default.
• W95593920 hasRelatedWork W10786496 @default.
• W95593920 hasRelatedWork W2406720631 @default.
• W95593920 isParatext "false" @default.
• W95593920 isRetracted "false" @default.
• W95593920 magId "95593920" @default.
• W95593920 workType "article" @default.