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- W958001834 abstract "A comparative study was conducted to focus on immune response level in different groups assist by some immunologic parameters in mice immunized with the whole cell killed yeast Cryptococcus neoformans vaccine. All experiments were carried out on a total number of120 male and female albino mice (Blab-c), mice were divided into equal three groups, the first group received distilled water as (control group), the second group was immunized with killed yeast Cryptococcus neoformans antigen and the third group was immunized with a life yeast Cryptococcus neoformans antigen. Vaccine efficiency was evaluated according to phagocytic activity percentage, delayed type hypersensitivity reaction test, anti-C.neoformans antibodies titer level and gamma globulin fraction percentage in immunized mice serum. All treatments were carried out on day one. Then the mice were scarified and tested at different periods: day 10, phagocytic activity percentage by Nitro Blue Tetrazolium test (NBT) was evaluated, at days 14 tests for delayed hypersensitivity type reaction of skin, and at day 21 and 28 performed the test for anti-anti-C.neoformans antibodies titer level in mice serum by indirect immunoflourescent assay and gamma globulin fraction percentage by Gel electrophoresis. Results revealed that third group recorded significantly higher values in their peripheral blood phagocytic activity by nitro blue tetrazolium test measured by ELISA reader and anti- C.neoformans antibodies titer level in mice serum at 21 and 28 days by indirect immunoflourescent assay and gamma globulin fraction as compared with control group. Second group results revealed significantly increased in phagocytic activity index of peripheral blood by nitro blue tetrazolium test and the anti- C.neoformans antibodies titer level assessed by indirect immunoflourescent test and gamma globulin fraction in mice serum at 21 and 28 days. In delayed-type hypersensitivity reaction, the index was significantly increased in group III vaccinated mice and in group II as comparison with control group, the best results was observed after 24 hours post-C.neoformans protein injection. Result concluded that a life C.neoformans antigen produce the best immune response in mice." @default.
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- W958001834 date "2015-01-01" @default.
- W958001834 modified "2023-09-27" @default.
- W958001834 title "Immunologic Study in Mice Immunized with Whole Cryptococcus Neoformans Fungusorganism" @default.
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