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- W958397324 abstract "This chapter reviews that mechanistic studies are motivated by in vivo observations of high-fidelity replication and repair of DNA and highly specific initiation, elongation, and termination of RNA transcription and by the belief that positive and negative regulators of both these aspects of gene expression must act, by altering in some fashion, the underlying reaction sequence, catalyzed by the polymerases involved. It discusses that kinetic studies, in parallel with product analysis and structural information, are an essential component of a complete biochemical description of these enzyme reaction mechanisms. It discusses how the processivity of a polymerase affects the interpretation of other kinetic parameters. It describes a new method for quantitatively, determining the processivity of a DNA polymerase, and describes the method, with data obtained, employing Escherichia coli DNA polymerase I. The chapter also discusses processivity in connection with other kinetic factors that affect the fidelity of nucleic acid polymerization." @default.
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- W958397324 date "1980-01-01" @default.
- W958397324 modified "2023-09-23" @default.
- W958397324 title "[11] The kinetics and processivity of nucleic acid polymerases" @default.
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- W958397324 doi "https://doi.org/10.1016/s0076-6879(80)64013-0" @default.
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