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• W958902652 endingPage "42" @default.
• W958902652 startingPage "33" @default.
• W958902652 abstract "Cells generate and experience mechanical forces that may shape tissues and regulate signaling pathways in a variety of physiological or pathological situations. How forces propagate and transduce signals at the molecular level is poorly understood. The advent of FRET-based Molecular Tension Microscopy now allows to achieve mechanical force measurements at a molecular scale with molecular specificity in situ, and thereby better understand the mechanical architecture of cells and tissues, and mechanotransduction pathways. In this review, we will first expose the basic principles of FRET-based MTM and its various incarnations. We will describe different ways of measuring FRET, their advantages and drawbacks. Then, throughout the range of proteins of interest, cells and organisms to which it has been applied, we will review the tests developed to validate the approach, how molecular tension was related to cell functions, and conclude with possible developments and offshoots." @default.
• W958902652 created "2016-06-24" @default.
• W958902652 creator A5011169804 @default.
• W958902652 creator A5030824131 @default.
• W958902652 date "2016-02-01" @default.
• W958902652 modified "2023-10-15" @default.
• W958902652 title "FRET-based Molecular Tension Microscopy" @default.
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• W958902652 doi "https://doi.org/10.1016/j.ymeth.2015.07.010" @default.
• W958902652 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26210398" @default.
• W958902652 hasPublicationYear "2016" @default.
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