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- W959098420 abstract "Publisher Summary This chapter describes the procedure for the preparation of apopyruvate decarboxylase. The preparation of apopyruvate decarboxylase depends on the ability of a small-pore molecular sieve to remove thiamine pyrophosphate (TPP) from apoprotein after the separation of TPP from the holoprotein at alkaline pH. Earlier methods used the alkaline washing of yeast or alkaline treatment of pyruvate decarboxylase followed by repeated ammonium sulfate precipitation to obtain the apoenzyme. The method described in the chapter is less harsh on the enzyme, more effective, and less time-consuming." @default.
- W959098420 created "2016-06-24" @default.
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- W959098420 date "1970-01-01" @default.
- W959098420 modified "2023-09-27" @default.
- W959098420 title "[20] Preparation of apopyruvate decar☐ylase (2-Oxoacid car☐y-lyase, EC 4.1.1.1)" @default.
- W959098420 cites W2132694049 @default.
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- W959098420 doi "https://doi.org/10.1016/0076-6879(71)18288-2" @default.
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