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- W960268231 abstract "分离得到1株产生淀粉酶的菌株,通过扩增和测定16S rDNA序列并进行比对,发现是Paenibacillus属的细菌。液体摇瓶发酵结束后,其产生的生淀粉酶比酶活达108.5U/mL。通过饱和（NH4）2SO4沉淀、Sephacryl S-300层析的方法对其所产的生淀粉酶进行分离纯化,得到纯化的酶蛋白比酶活为5112.04U/mg,纯化倍数为14.1,相对分子质量约为1.0×105。该酶以木薯生淀粉为底物时,最适pH5.6,最适温度50℃。金属离子Ca2＋、Mg2＋对该酶具有激活作用,Zn2＋、Cu2＋、Fe2＋、Ni2＋、Mn2＋、Co2＋和EDTA2-对该酶均具有抑制作用。" @default.
- W960268231 created "2016-06-24" @default.
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- W960268231 date "2010-01-01" @default.
- W960268231 modified "2023-09-23" @default.
- W960268231 title "生淀粉酶生产菌株Paenibacillus sp.的筛选和酶的纯化及酶学性质" @default.
- W960268231 hasPublicationYear "2010" @default.
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