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- W960344755 abstract "Publisher Summary The method of isoelectric focusing is making new inroads in the study of proteins from various sources. The method requires a stable pH gradient between the anode and the cathode in an electrolysis cell. This is obtained by the electrolysis of a water solution of a mixture of suitable low molecular weight ampholytes, called carrier ampholytes. When proteins are put into such a system each protein migrates to, and focuses at, its isoelectric point, p I , independent of where it was put into the apparatus at the start. Therefore, the volume of the protein sample solution to be applied is not critical and even dilute protein solutions of comparatively large volumes can be applied. Isoelectric focusing requires some means to counteract convective remixing of the separating components. At present three stabilizing techniques are used—(1) density gradient, (2) polyacrylamide-gel, (3) zone convection electrofocusing. The first technique is the most common, and an apparatus for 110 ml and another for 440 ml are commercially available. The second is of rapidly growing importance and is especially valuable for analytical separations and comparative work. The third technique is very simple, as it is in principle performed in a serpentine channel and does not require such additives as sucrose or gel." @default.
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- W960344755 date "1971-01-01" @default.
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- W960344755 title "[33] Isoelectric focusing of proteins" @default.
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