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- W96073248 abstract "This study aims at understanding the role of the UvrD helicase in the restart of arrested replication forks in Escherichia coli. After replication arrest at ectopic replication terminaison sites of Ter/Tus, we showed that UvrD is the major helicase needed to restart. We propose that UvrD acts in concert with homologous recombination proteins to dislodge Tus form Ter sites during replication restart. The Tus-removal action of UvrD is conserved in Bacillus subtilis homologous helicase PcrA. After replication fork arrest by the inactivation of a subunit of the DNA polymerase III holoenzyme (Pol Illh), either the catalytic subunit (dnaEts mutant) or the (3-clamp (dnaNts mutant), the anti-RecA action of UvrD at blocked forks is essential for the replication fork reversal reaction (RPR) to promote replication restart. We have shown that the anti-RecA action of UvrD at blocked forks reflects two different activities of this enzyme. An ATPase-deficient UvrD mutant is able to antagonize RecA in cells affected for the Pol IIIh catalytic subunit DnaE. In this mutant, RecA action at blocked forks specifically requires the protein RarA (MgsA). This suggests that UvrD acts by preventing RecA binding, possibly through counteracting RarA. In contrast, at forks affected for the Pol Illh clamp (DnaN), RarA is not required for RecA binding and the ATPase fonction of UvrD is essential to counteract RecA, supporting the idea that UvrD removes RecA from DNA. The anti-RecA action of UvrD at Pol IIIts-blocked forks is conserved in the Bacillus subtilis homologous helicase PcrA. Proliferative disorders, this unique mutation will permit a new molecular classification of these disorders and novel therapeutical approaches." @default.
- W96073248 created "2016-06-24" @default.
- W96073248 creator A5058015886 @default.
- W96073248 date "2008-01-01" @default.
- W96073248 modified "2023-09-23" @default.
- W96073248 title "Action de l'hélicase UvrD lors du redémarrage des fourches de réplication chez la bactérie Escherichia coli" @default.
- W96073248 hasPublicationYear "2008" @default.
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