Matches in SemOpenAlex for { <https://semopenalex.org/work/W96096802> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W96096802 endingPage "312" @default.
- W96096802 startingPage "299" @default.
- W96096802 abstract "A DNA microarray-based approach is described for screening metagenomic libraries for the presence of selected genes. The protocol is exemplified for the identification of flavin-binding, blue-light-sensitive biological photoreceptors (BL), based on a homology search in already sequenced, annotated genomes. The microarray carried 149 different 54-mer oligonucleotides, derived from consensus sequences of BL photoreceptors. The array could readily identify targets carrying 4% sequence mismatch, and allowed unambiguous identification of a positive cosmid clone of as little as 10 ng against a background of 25 μg of cosmid DNA. The protocol allows screening up to 1,200 library clones in concentrations as low as ca. 20 ng, each with a ca. 40 kb insert size readily in a single batch. Calibration and control conditions are outlined. This protocol, when applied to the thermophilic fraction of a soil sample, yielded the identification and functional characterization of a novel, BL-encoding gene that showed a 58% similarity to a known, BL-encoding gene from Kineococcus radiotolerans SRS30216 (similarity values refer to the respective LOV domains)." @default.
- W96096802 created "2016-06-24" @default.
- W96096802 creator A5040909240 @default.
- W96096802 creator A5082525055 @default.
- W96096802 date "2010-01-01" @default.
- W96096802 modified "2023-09-25" @default.
- W96096802 title "Detection and Isolation of Selected Genes of Interest from Metagenomic Libraries by a DNA Microarray Approach" @default.
- W96096802 cites W1963867445 @default.
- W96096802 cites W2014167027 @default.
- W96096802 cites W2065062695 @default.
- W96096802 cites W2088145350 @default.
- W96096802 cites W2129631787 @default.
- W96096802 cites W2145132040 @default.
- W96096802 cites W2146684826 @default.
- W96096802 cites W2154218487 @default.
- W96096802 cites W2166940906 @default.
- W96096802 doi "https://doi.org/10.1007/978-1-60761-823-2_21" @default.
- W96096802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20830573" @default.
- W96096802 hasPublicationYear "2010" @default.
- W96096802 type Work @default.
- W96096802 sameAs 96096802 @default.
- W96096802 citedByCount "2" @default.
- W96096802 countsByYear W960968022013 @default.
- W96096802 countsByYear W960968022021 @default.
- W96096802 crossrefType "book-chapter" @default.
- W96096802 hasAuthorship W96096802A5040909240 @default.
- W96096802 hasAuthorship W96096802A5082525055 @default.
- W96096802 hasConcept C104317684 @default.
- W96096802 hasConcept C111824103 @default.
- W96096802 hasConcept C129312508 @default.
- W96096802 hasConcept C13575015 @default.
- W96096802 hasConcept C141231307 @default.
- W96096802 hasConcept C1436000 @default.
- W96096802 hasConcept C150194340 @default.
- W96096802 hasConcept C15151743 @default.
- W96096802 hasConcept C153911025 @default.
- W96096802 hasConcept C167625842 @default.
- W96096802 hasConcept C2781188995 @default.
- W96096802 hasConcept C35101798 @default.
- W96096802 hasConcept C42062724 @default.
- W96096802 hasConcept C51679486 @default.
- W96096802 hasConcept C54355233 @default.
- W96096802 hasConcept C552990157 @default.
- W96096802 hasConcept C70721500 @default.
- W96096802 hasConcept C86803240 @default.
- W96096802 hasConcept C95371953 @default.
- W96096802 hasConceptScore W96096802C104317684 @default.
- W96096802 hasConceptScore W96096802C111824103 @default.
- W96096802 hasConceptScore W96096802C129312508 @default.
- W96096802 hasConceptScore W96096802C13575015 @default.
- W96096802 hasConceptScore W96096802C141231307 @default.
- W96096802 hasConceptScore W96096802C1436000 @default.
- W96096802 hasConceptScore W96096802C150194340 @default.
- W96096802 hasConceptScore W96096802C15151743 @default.
- W96096802 hasConceptScore W96096802C153911025 @default.
- W96096802 hasConceptScore W96096802C167625842 @default.
- W96096802 hasConceptScore W96096802C2781188995 @default.
- W96096802 hasConceptScore W96096802C35101798 @default.
- W96096802 hasConceptScore W96096802C42062724 @default.
- W96096802 hasConceptScore W96096802C51679486 @default.
- W96096802 hasConceptScore W96096802C54355233 @default.
- W96096802 hasConceptScore W96096802C552990157 @default.
- W96096802 hasConceptScore W96096802C70721500 @default.
- W96096802 hasConceptScore W96096802C86803240 @default.
- W96096802 hasConceptScore W96096802C95371953 @default.
- W96096802 hasLocation W960968021 @default.
- W96096802 hasLocation W960968022 @default.
- W96096802 hasOpenAccess W96096802 @default.
- W96096802 hasPrimaryLocation W960968021 @default.
- W96096802 hasRelatedWork W1847307531 @default.
- W96096802 hasRelatedWork W2014982185 @default.
- W96096802 hasRelatedWork W2099098650 @default.
- W96096802 hasRelatedWork W2140008189 @default.
- W96096802 hasRelatedWork W2147312682 @default.
- W96096802 hasRelatedWork W2153695430 @default.
- W96096802 hasRelatedWork W2170927484 @default.
- W96096802 hasRelatedWork W2262251148 @default.
- W96096802 hasRelatedWork W2984906837 @default.
- W96096802 hasRelatedWork W96096802 @default.
- W96096802 isParatext "false" @default.
- W96096802 isRetracted "false" @default.
- W96096802 magId "96096802" @default.
- W96096802 workType "book-chapter" @default.