FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W96133764> ?p ?o ?g. }

• W96133764 abstract "The purpose of this project was to investigate techniques that may be successfully applied to improve the characteristics of a bioprocess relevant mammalian cell line such as CHO (Chinese Hamster Ovary).The first technique explored in this thesis investigates the possibility of gene targeting and knockout using an AAV (adeno associated virus) viral vector. A custom vector was constructed comprised of an insertion cassette flanked by homologous ‘arm’ sequences generated from the region of genomic DNA being targeted. Infecting cells with this virus at an MOI of over 2 x 105 particles per cell, we have demonstrated that this unique genomic locus can be successfully targeted with foreign DNA through homologous recombination in CHO cells, with a targeting frequency of 3.1% calculated in this instance. While single allele knockouts were detected using a DHFR targeting vector, a number of alternative techniques implemented did not successfully isolate homozygous negative mutants, however further work based on the data provided in this study could successfully generate null mutants.The second technique applied is a variation of the directed evolution approach. A gene (TBP) was first selected that is known to be involved at a high level in the global cellular transcriptional machinery and subjected to random mutagenesis to generate a mutant library. When transfected this created a heterogeneous pool of diverse clones with a variety of phenotypes. An environmental stress was then applied for a number of passages to select for clones with suitable advantageous mutations, which were then isolated and characterised. Using this technique we successfully isolated a number of mutant clones with average maximum viable cell densities 53% greater than a control cell line when placed through the same stress selective procedure. This improvement in growth coupled with additional benefits to late culture stage viability contributed to an increase in accumulated integrated viable cell density (viable cell-hours) of over 44%. A transcriptional profiling experiment was also carried out to investigate the underlying cellular mechanisms that may have contributed to these advantageous metabolic changes." @default.
• W96133764 created "2016-06-24" @default.
• W96133764 creator A5036691873 @default.
• W96133764 date "2013-11-01" @default.
• W96133764 modified "2023-09-27" @default.
• W96133764 title "Mutagenesis and directed evolution in CHO cells to improve bio-production characteristics" @default.
• W96133764 cites W1508733793 @default.
• W96133764 cites W1547660323 @default.
• W96133764 cites W1582366477 @default.
• W96133764 cites W1611419331 @default.
• W96133764 cites W1639694146 @default.
• W96133764 cites W1795995412 @default.
• W96133764 cites W1860242155 @default.
• W96133764 cites W1933088939 @default.
• W96133764 cites W1964907714 @default.
• W96133764 cites W1967156547 @default.
• W96133764 cites W1969935644 @default.
• W96133764 cites W1981587090 @default.
• W96133764 cites W1982234546 @default.
• W96133764 cites W1989932051 @default.
• W96133764 cites W1993712998 @default.
• W96133764 cites W2012175027 @default.
• W96133764 cites W2016786878 @default.
• W96133764 cites W2021887610 @default.
• W96133764 cites W2028228274 @default.
• W96133764 cites W2031066980 @default.
• W96133764 cites W2031409259 @default.
• W96133764 cites W2039618053 @default.
• W96133764 cites W2042059381 @default.
• W96133764 cites W2047511535 @default.
• W96133764 cites W2050921552 @default.
• W96133764 cites W2051442157 @default.
• W96133764 cites W2055863441 @default.
• W96133764 cites W2056026468 @default.
• W96133764 cites W2062958450 @default.
• W96133764 cites W2063852412 @default.
• W96133764 cites W2068314225 @default.
• W96133764 cites W2069284062 @default.
• W96133764 cites W2071995179 @default.
• W96133764 cites W2074631636 @default.
• W96133764 cites W2075218265 @default.
• W96133764 cites W2076584115 @default.
• W96133764 cites W2077585895 @default.
• W96133764 cites W2079397645 @default.
• W96133764 cites W2080537888 @default.
• W96133764 cites W2082964247 @default.
• W96133764 cites W2083393088 @default.
• W96133764 cites W2084046616 @default.
• W96133764 cites W2087823077 @default.
• W96133764 cites W2087952915 @default.
• W96133764 cites W2092313455 @default.
• W96133764 cites W2095612833 @default.
• W96133764 cites W2100475769 @default.
• W96133764 cites W2102279520 @default.
• W96133764 cites W2102654270 @default.
• W96133764 cites W2109918485 @default.
• W96133764 cites W2112091114 @default.
• W96133764 cites W2113446079 @default.
• W96133764 cites W2115124797 @default.
• W96133764 cites W2119161392 @default.
• W96133764 cites W2123502778 @default.
• W96133764 cites W2131566517 @default.
• W96133764 cites W2131905022 @default.
• W96133764 cites W2137347518 @default.
• W96133764 cites W2140937256 @default.
• W96133764 cites W2144011039 @default.
• W96133764 cites W2146603022 @default.
• W96133764 cites W2146901740 @default.
• W96133764 cites W2148388239 @default.
• W96133764 cites W2148590972 @default.
• W96133764 cites W2151091554 @default.
• W96133764 cites W2161463357 @default.
• W96133764 cites W2162535410 @default.
• W96133764 cites W2165276945 @default.
• W96133764 cites W2168245648 @default.
• W96133764 cites W2168928403 @default.
• W96133764 cites W2254057196 @default.
• W96133764 cites W2291321734 @default.
• W96133764 cites W238668910 @default.
• W96133764 hasPublicationYear "2013" @default.
• W96133764 type Work @default.
• W96133764 sameAs 96133764 @default.
• W96133764 citedByCount "0" @default.
• W96133764 crossrefType "dissertation" @default.
• W96133764 hasAuthorship W96133764A5036691873 @default.
• W96133764 hasConcept C100012643 @default.
• W96133764 hasConcept C102744134 @default.
• W96133764 hasConcept C104317684 @default.
• W96133764 hasConcept C111829913 @default.
• W96133764 hasConcept C143065580 @default.
• W96133764 hasConcept C16318435 @default.
• W96133764 hasConcept C175656101 @default.
• W96133764 hasConcept C25365240 @default.
• W96133764 hasConcept C40767141 @default.
• W96133764 hasConcept C54009773 @default.
• W96133764 hasConcept C54355233 @default.
• W96133764 hasConcept C70721500 @default.
• W96133764 hasConcept C77957584 @default.
• W96133764 hasConcept C81885089 @default.
• W96133764 hasConcept C86803240 @default.

• next