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- W961925433 abstract "Objective To analyze the melocular features of N,G gene of rabies virus in Beijing and disclose the differences of this virulent strains in Beijing from other epidemic strains and vaccine strains in other provinces. Methods A rabies virus was collected from a suspected patient, its nucleoprotein and glycoprotein genes were analyzed. Rabies virus antigens were detected from the patient's brain by direct immunofluorescence, and the isolate named as Beijing (H) was obtained by inoculating the suspension of positive brains into suckling mice. The N and G genes of the rabies virus were amplified by RT-PCR,and then sequenced. Rersults Genetic analysis indicated that the strain was highly homologous to the street viruses endemic in China with the a homology of 97.6%～99.1% and 97.5%～99.7% respectively were observed in comparison of N and G genes with those street viruses; and the deduced amino acid's homology was 97.6%～99.2% and 97.7%～99.8%, respectively. Conlcusion The isolate Beijing(H) belonged to classic rabies virus, and shared a high homology with the street virus from dogs in the main endemic areas in China. The most important is that both N and G genes diverged from the current vaccine strains used in China at either the nucleotide or the amino acid level." @default.
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- W961925433 date "2009-01-01" @default.
- W961925433 modified "2023-09-27" @default.
- W961925433 title "Identification and genetic analysis of a rabies virus strain carried by a patient in Beijing." @default.
- W961925433 hasPublicationYear "2009" @default.
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