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- W962685355 abstract "目的：探讨在染料木黄酮（genistein,Gen）与氟尿嘧啶（5-fluorouracil,5-FU）诱导肝癌MHCC97-L细胞凋亡作用中p42/44丝裂原活化蛋白激酶（mitogen-activated protein kinases,MAPKs）对Bax的调控.方法：倒置显微镜观察细胞密度和细胞形态;荧光显微镜（Annexin V-FITC/PI）检测细胞凋亡及细胞死亡;Western blot检测Bax蛋白表达.结果：倒置显微镜下,所有药物组细胞密度均下降,死亡细胞及碎片散落在培养液中,贴壁细胞皱缩或变形,胞浆透明度下降,可见空泡结构,其中以PD98059＋Gen＋5-FU组变化最明显;荧光显微镜下,所有药物组均可检测到凋亡和死亡细胞;Western blot检测各组Bax蛋白的相对表达量由高到低依次为Gen＋5-F U、G e n、5-F U、P D98059＋G e n＋5-F U、PD98059＋5-FU、PD98059＋Gen、PD98059、对照组,PD98059＋Gen组的Bax蛋白表达与P D98059组十分接近,且明显低于G e n组;PD98059＋5-FU组和PD98059＋Gen＋5-FU组的Bax蛋白表达介于单用抑制剂组与未用抑制剂预处理的对应药物组之间.结论：Gen、5-FU和Gen＋5-FU均能诱导MHCC97-L细胞凋亡,并与上调Bax有关;Gen对Bax的调控与p42/44 MAPK通路关系密切;5-FU和联用组调控Bax需要有p42/44 MAPK通路参与." @default.
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- W962685355 date "2014-01-01" @default.
- W962685355 modified "2023-09-27" @default.
- W962685355 title "Genistein and 5-FU inhibit apoptosis of human hepatocellular cancer cells by regulating Bax via p42/44 MAPK pathway" @default.
- W962685355 doi "https://doi.org/10.11569/wcjd.v22.i14.2003" @default.
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