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- W964381010 abstract "This chapter describes the colorimetric ultramicro assay for reducing sugars. Ferrocyanide produced as the result of the reduction of ferricyanide by a reducing sugar is assayed indirectly by the formation of ferrous ions. These subsequently interact with an iron chelate to yield a product with high color intensity. The reagent for iron employed in the following procedure is 2,4,6-tripyridyl-s-triazine. Reactions should be shielded from direct light. Aliquots (1.0 ml) of solutions containing 2–100 nmoles of reducing sugar are placed in test tubes. Reagent A (1.0 ml) is added, and the tubes are kept for 10 min in a boiling-water bath. Reagent B (1.0 ml) and reagent C (2.0 ml) are then added immediately, without cooling. The solutions are stirred vigorously with a Vibromixer, after 5 min in the dark, the absorbance at 595 nm of the violet Fe (TPTZ) 2+2 is against a reagent blank. It has been found out that 3-(2-pyridyl)-5,6-diphenyl-l,2,4-triazine can substitute for TPTZ as the reagent for ferrous ions used for the determination of reducing sugars. A procedure for the determination of ferrocyanide by direct UV-spectrophotometry in an assay for reducing sugars has been described. This method may be employed only when no components other than ferrocyanide that adsorb significantly in the far ultraviolet range are present in the solution analyzed." @default.
- W964381010 created "2016-06-24" @default.
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- W964381010 date "1975-01-01" @default.
- W964381010 modified "2023-10-17" @default.
- W964381010 title "[4] Colorimetric ultramicro assay for reducing sugars" @default.
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- W964381010 doi "https://doi.org/10.1016/s0076-6879(75)41006-0" @default.
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