Matches in SemOpenAlex for { <https://semopenalex.org/work/W965856777> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W965856777 endingPage "60" @default.
- W965856777 startingPage "55" @default.
- W965856777 abstract "We have developed an efficient serum free culture model for cloning human erythroid progenitors. Accordingly, human bone marrow or cord blood CD34+ cells if plated in our serum free medium and stimulated with a mixture of EpO + KL, grow erythroid colonies exclusively. Cells isolated from these cultures express glycophorin-A (GPA-A), are CD33-, IIb/IIIa-, and finally all become hemoglobinized. By employing this system we also found out that cord blood CD34+ mononuclear cells (MNC) contain more BFU-E than adult marrow CD34+ MNC, moreover, the erythroid colonies formed by cord blood progenitors are significantly larger then the ones formed by the marrow cells. We have also compared the influence of different cytokines and growth factors, which were reported in the literature to costimulate BFU-E growth on cloning efficiency of human BFU-E cultured in our serum free medium. We found that from 20 different growth factors and cytokines tested, EpO dependent bone marrow BFU-E growth is costimulated only by KL, and to lesser degree also by IL-3, GM-CSF, TpO and IL-9. In contrast to marrow cells we observed that cord blood BFU-E in addition to KL, IL-3, GM-CSF, TpO, LIF and IL-9 were also costimulated by NGF-beta, FGF-1, FGF-2 and STK-IL. We found simultaneously that TPO which possess only negligible costimulatory effect on erythroid colony formation by bone marrow CD34+ cells, significantly costimulated the formation of erythroid colonies grown by cord blood CD34+ cells. Therefore, the cord blood CD34+ cells are largely committed to erythroid differentiation, and, moreover, they respond to a wider spectrum of the growth factors than their bone marrow counterparts." @default.
- W965856777 created "2016-06-24" @default.
- W965856777 creator A5021797350 @default.
- W965856777 creator A5026426787 @default.
- W965856777 creator A5028413682 @default.
- W965856777 creator A5040218043 @default.
- W965856777 creator A5060689813 @default.
- W965856777 creator A5076627630 @default.
- W965856777 date "1998-01-01" @default.
- W965856777 modified "2023-10-05" @default.
- W965856777 title "An improved serum free system for cloning human pure erythroid colonies. The role of different growth factors and cytokines on BFU-E formation by the bone marrow and cord blood CD34+ cells." @default.
- W965856777 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9606618" @default.
- W965856777 hasPublicationYear "1998" @default.
- W965856777 type Work @default.
- W965856777 sameAs 965856777 @default.
- W965856777 citedByCount "1" @default.
- W965856777 crossrefType "journal-article" @default.
- W965856777 hasAuthorship W965856777A5021797350 @default.
- W965856777 hasAuthorship W965856777A5026426787 @default.
- W965856777 hasAuthorship W965856777A5028413682 @default.
- W965856777 hasAuthorship W965856777A5040218043 @default.
- W965856777 hasAuthorship W965856777A5060689813 @default.
- W965856777 hasAuthorship W965856777A5076627630 @default.
- W965856777 hasConcept C10205521 @default.
- W965856777 hasConcept C109159458 @default.
- W965856777 hasConcept C137061746 @default.
- W965856777 hasConcept C153911025 @default.
- W965856777 hasConcept C201750760 @default.
- W965856777 hasConcept C202751555 @default.
- W965856777 hasConcept C203014093 @default.
- W965856777 hasConcept C2778690821 @default.
- W965856777 hasConcept C2780007613 @default.
- W965856777 hasConcept C2780914630 @default.
- W965856777 hasConcept C28328180 @default.
- W965856777 hasConcept C55493867 @default.
- W965856777 hasConcept C86803240 @default.
- W965856777 hasConcept C95444343 @default.
- W965856777 hasConceptScore W965856777C10205521 @default.
- W965856777 hasConceptScore W965856777C109159458 @default.
- W965856777 hasConceptScore W965856777C137061746 @default.
- W965856777 hasConceptScore W965856777C153911025 @default.
- W965856777 hasConceptScore W965856777C201750760 @default.
- W965856777 hasConceptScore W965856777C202751555 @default.
- W965856777 hasConceptScore W965856777C203014093 @default.
- W965856777 hasConceptScore W965856777C2778690821 @default.
- W965856777 hasConceptScore W965856777C2780007613 @default.
- W965856777 hasConceptScore W965856777C2780914630 @default.
- W965856777 hasConceptScore W965856777C28328180 @default.
- W965856777 hasConceptScore W965856777C55493867 @default.
- W965856777 hasConceptScore W965856777C86803240 @default.
- W965856777 hasConceptScore W965856777C95444343 @default.
- W965856777 hasIssue "2" @default.
- W965856777 hasLocation W9658567771 @default.
- W965856777 hasOpenAccess W965856777 @default.
- W965856777 hasPrimaryLocation W9658567771 @default.
- W965856777 hasRelatedWork W1569098572 @default.
- W965856777 hasRelatedWork W1812483684 @default.
- W965856777 hasRelatedWork W2063113696 @default.
- W965856777 hasRelatedWork W2322667139 @default.
- W965856777 hasRelatedWork W2358325675 @default.
- W965856777 hasRelatedWork W2391166179 @default.
- W965856777 hasRelatedWork W2394295848 @default.
- W965856777 hasRelatedWork W2416649916 @default.
- W965856777 hasRelatedWork W2531493050 @default.
- W965856777 hasRelatedWork W2946940121 @default.
- W965856777 hasVolume "36" @default.
- W965856777 isParatext "false" @default.
- W965856777 isRetracted "false" @default.
- W965856777 magId "965856777" @default.
- W965856777 workType "article" @default.