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- W965862419 abstract "通过对下载的16株小反刍兽疫病毒（PPRV）N基因片段进行序列分析,合成了PPRV N基因。用NP1和NP2引物扩增。纯化的PPRV N基因在T4DNA连接酶的作用下,和PET32-a载体进行连接反应构建重组质粒pET-a-PPRV-N。经诱导表达和纯化后,得到了大量的PPRV N蛋白。用PPRV N蛋白制备免疫血清,经国际标准血清标化后作为参考阳性血清,同时以PPRV N蛋白为抗原建立间接ELISA（I-ELISA）方法。对山东省的467份羊血清检测,使用S/P（样品值/阳性值）平均值加3倍标准差的方法,计算出其临界点为0.28。应用间接ELISA和针对H蛋白的单抗为基础的C-ELISA检测来自于疫区的69份血清,结果两者的符合率为79.7%。" @default.
- W965862419 created "2016-06-24" @default.
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- W965862419 date "2008-01-01" @default.
- W965862419 modified "2023-09-24" @default.
- W965862419 title "小反刍兽疫病毒（PPRV）N蛋白的表达与ELISA检测方法的建立" @default.
- W965862419 hasPublicationYear "2008" @default.
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