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- W966160512 abstract "目的：观察透骨消痛胶囊含药血清对软骨细胞CyclinD1 mRNA表达的影响,探讨透骨消痛胶囊治疗骨性关节炎的可能作用机制。方法：采用抽签法将16只12周龄雄性新西兰大白兔随机分为空白组、低剂量组、中剂量组、高剂量组,每组4只。低剂量组按5 mL.kg-1体质量以5 mg.mL-1透骨消痛胶囊溶液灌胃,中剂量组按5 mL.kg-1体质量以10 mg.mL-1透骨消痛胶囊溶液灌胃,高剂量组按5 mL.kg-1体质量以20 mg.mL-1透骨消痛胶囊溶液灌胃,空白组按5 mL.kg-1体质量以生理盐水灌胃。每天灌胃2次,连续7 d,最后1 d连续2次灌胃,中间间隔2 h。末次灌胃后3 h提取各组实验兔含药血清低温保存备用。将6只4周龄雄性新西兰兔处死,取其膝关节软骨,置入盛有Ⅱ型胶原酶的培养皿中进行消化,建立软骨细胞体外培养体系,并采用甲苯胺蓝染色法鉴定细胞功能。将体外培养的第3代软骨细胞随机分为空白组、低剂量组、中剂量组、高剂量组,并分别加入含空白血清、低剂量中药血清、中剂量中药血清、高剂量中药血清的DMEM培养液中继续培养72 h后,采用MTT法检测软骨细胞的增殖情况,采用RT-PCR法检测CyclinD1 mRNA的表达,并在透射电子显微镜下观察细胞形态。结果：Ⅱ型胶原酶消化法成功建立了软骨细胞的体外培养体系,甲苯胺蓝染色可见软骨细胞内呈紫红色异染颗粒。干预72 h后,各组软骨细胞光密度值比较,差异有统计学意义（F=6.209,P=0.004）;中剂量组、高剂量组软骨细胞光密度值高于空白组（P=0.005,P=0.002）;中剂量组、高剂量组软骨细胞光密度值高于低剂量组（P=0.034,P=0.011）。各组软骨细胞CyclinD1 mRNA表达量比较,差异有统计学意义（F=8.262,P=0.001）;中剂量组、高剂量组软骨细胞CyclinD1 mRNA表达量高于空白组（P=0.002,P=0.001）;中剂量组、高剂量组软骨细胞CyclinD1 mRNA表达量高于低剂量组（P=0.012,P=0.004）;中剂量�" @default.
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- W966160512 date "2012-01-01" @default.
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- W966160512 title "透骨消痛胶囊含药血清对软骨细胞Cyclin D1 mRNA表达的影响" @default.
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