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- W9663243 abstract "IL-6 is a pleiotropic cytokine, being both a mitogen and inhibitor of apoptosis. Involvement of IL-6 in various diseases including carcinogenesis has been established. The aim of this study was to determine role of IL-6 expression and secretion on Cox-2 in activated HET-1 cells. HET-1 cell cultures were activated with growth media containing IL-6 (100U/ml) or acidified growth media (pH 4.5). Pharmacologic inhibitors including NS398 (Cox-2), Wortmannin (PI3k/Akt), SB203580 (p38 MAPK) and PD098059 (MEK/ERK) were used to identify the signaling pathways involving IL-6 and Cox-2. Following activation, the cells were rinsed to remove any residual activators and inhibitors, and standard growth media was replaced. IL-6 and PGE2 production in culture media was determined by ELISA. mRNA and protein expression were determined by RT-PCR and Western blotting. Both acidic pH and exogenous IL-6 enhanced IL-6 production. Wortmannin did not affect IL-6 secretion, whereas NS398 further enhanced IL-6 production in both activated conditions. Acidic pH also enhanced PGE2 production, which was inhibited by NS398. Conversely, exogenous IL-6 exerted an unexpected inhibition of PGE2, and NS398 led to a further unexpected reversal of the inhibition. Wortmannin did not affect PGE2 secretion in either activated condition. These data demonstrate a possible novel pathway mechanism between IL-6 and Cox-2 not previously observed. Supported by Digestive Disease Center and Cancer Center (P.R., D.G.B.)" @default.
- W9663243 created "2016-06-24" @default.
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- W9663243 date "2006-03-01" @default.
- W9663243 modified "2023-09-27" @default.
- W9663243 title "IL‐6 expression and secretion in HET‐1 cells is associated with Cox‐2" @default.
- W9663243 doi "https://doi.org/10.1096/fasebj.20.5.a1079-d" @default.
- W9663243 hasPublicationYear "2006" @default.
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