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- W966596488 abstract "应用分子生物学技术，建立顺序特异引物聚合酶链反应方法，首次对１１２例肾移植供受者和９份标准ＤＮＡ进行ＨＬＡ－ＤＲ基因分型，每份样本同时２０管ＰＣＲ的反应，扩增条件为９４℃３０秒，６０℃５０秒，７２℃４０秒，共３４个循环，即可对ＤＲ各等位基因作出准确分型，总耗时５小时。分型结果经标准ＤＮＡ，限制性内切酶分析和特异性探针杂交等予以确证，特异性和重复性１００％，无一例假阳性和假阴性，显示本方法快速、精确" @default.
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- W966596488 date "1996-01-22" @default.
- W966596488 modified "2023-09-25" @default.
- W966596488 title "PCR—SSP快速HLA—DR基因分型与临床应用" @default.
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