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- W966724828 abstract "This chapter discusses the HOHAHA or TOCSY, and with particular emphasis on the experimental details required for recording optimal spectra on biological samples. This chapter presents methods for recording the HOHAHA spectra in H2O solution are especially useful for connecting the side-chain protons with the usually better resolved amide resonances. At presence, most commercial spectrometers require some special hardware for producing the rf fields that have to be generated during the mixing period. Two-dimensional HOHAHA experiments are particularly useful for delineating individual spin systems in proteins. The method provides high-sensitivity, high-resolution spectra that contain a wealth of relayed connectivity information. There are two reasons HOHAHA spectroscopy may be more sensitive than conventional COSY-type experiments. First, because of the in-phase nature of the transfer of magnetization, cancellation of absorptive components within a cross-multiplet does not occur. Second, no magnetization is lost to the generation of spurious multiple-quantum coherence, a major source of sensitivity loss in conventional RELAY experiments. Resolution in the fingerprint region of double-quantum-filtered COSY and HOHAHA spectra is quite similar; although in the experience, the sensitivity of the HOHAHA spectra is typically much higher. For studying spectral regions close to the diagonal, the HOHAHA spectrum usually offers the best appearance because of the relatively low intensity of the diagonal." @default.
- W966724828 created "2016-06-24" @default.
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- W966724828 date "1989-01-01" @default.
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- W966724828 title "[8] Homonuclear Hartmann-Hahn experiments" @default.
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- W966724828 doi "https://doi.org/10.1016/0076-6879(89)76010-9" @default.
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