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- W966746471 abstract "Publisher Summary The technique of radioimmunoassay (RIA), which was used for the measurement of plasma insulin, has now been expanded to include the detection of minute amounts of a wide variety of substances of biological interest, including steroid hormones and drugs that are not antigenic per se. It has become the method of choice for the measurement of most protein and polypeptide hormones. RIA is a saturation assay technique, using the antibody as a specific binding agent. In this method, the antigen (Ag) to be measured competes with a labeled derivative for the binding sites of the antibody. After equilibration, the antigen bound to antibody is physically separated from the free antigen, and the label on the antigen either in the bound or free state is measured and compared with the corresponding value for standard solutions of the antigen. RIA procedures depend on the following prerequisites: (1) production of antisera with a high affinity and specificity for the antigen; (2) preparation of labeled antigens; (3) standardization procedures; (4) separation techniques; and (5) assay validation and expression of the results." @default.
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- W966746471 date "1975-01-01" @default.
- W966746471 modified "2023-09-27" @default.
- W966746471 title "Chapter 29. Radioimmunoassays" @default.
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