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- W966802303 abstract "[Objective] The research aimed to analyze the generated cause of vitrification phenomenon in tissue culture of eucalyptus, and explore the measures that would overcome or lessen vitrification phenomenon. [Method] With Guanglin 9 of eucalyptus grandis variety as tested material, the basic medium was: agar 3.0‰,sugar 30‰, NH4(superscript +) 5.0 ml, Ca(superscript 2+) 10 ml, and water was tap water, 11 formula treatments were set up aiming at the vitrification phenomenon occurred in tissue culture to study the effect of changing the contents of agar, sucrose, NH4(superscript +) and Ca(superscript 2+) and the water quality state in medium on conquering the vitrification in eucalyptus tissue culture.[Result] Some measures of overcoming or decreasing vitrification phenomenon in tissue culture of eucalyptus were proposed in the research as follows: generally, the suitable dosage of hardener was 3.5‰ when preparing medium; the sucrose content in medium could be increased about 10‰ properly to reduce the osmotic potential of medium and prevent culture material from absorbing moisture excessively; the Ca content in medium could be increased by 1.5 ml, while NH4(superscript +) content could be decreased by 2.0 mol/L; its better to use running water when cooking medium. [Conclusion] The study supplied scientific basis for overcoming vitrification phenomenon in tissue culture of eucalyptus." @default.
- W966802303 created "2016-06-24" @default.
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- W966802303 date "2009-09-20" @default.
- W966802303 modified "2023-09-23" @default.
- W966802303 title "桉树组织培养中的“玻璃化”现象及其克服措施" @default.
- W966802303 hasPublicationYear "2009" @default.
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