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- W966980212 abstract "This chapter discusses intracellular and intercellular pH measurement with microelectrodes. An important step has been the development of liquid ion-exchanger type microelectrodes. This microelectrode has a good performance with high selectivity for H + and a fast response time. It is relatively easy to make and has been used successfully to measure pH in higher plant cells. The chapter describes the construction of a pH microelectrode. To implant the microelectrodes in a cell under the microscope, a micromanipulator is required. There are several types of micromanipulators, most of which work mechanically using levers or screw action but at least one type works pneumatically and another by sliding one metal surface over another using thick grease as a lubricant. The aim is to reduce the movement of the operator's hand to microscopic proportions, and an instrument that can locate the tip of a microelectrode to 1μm or less is required." @default.
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- W966980212 date "1989-01-01" @default.
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- W966980212 title "[23] Intracellular and intercellular pH measurement with microelectrodes" @default.
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- W966980212 doi "https://doi.org/10.1016/0076-6879(89)74026-x" @default.
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