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- W967059400 abstract "This chapter discusses the methods used for the isolation of phosphorylated nonhistone proteins and techniques for studying their interaction with DNA. Phosphorylated proteins are a major component of the nonhistone (acidic) chromatin protein fraction of the cell nucleus, and many of their properties suggest that they play a role in the specific regulation of gene activity. For example, (1) changes in their phosphorylation correlate with changes in gene activity, (2) they are heterogeneous and tissue specific, (3) they increase the rate and type of RNA synthesis in cell-free systems, and (4)they can be shown to bind specifically to DNA. The method most commonly employed in the laboratory for the purification of these proteins involves salt extraction and selective purification on calcium phosphate gel. The chapter describes the procedure for rat liver, but it can be adapted to a wide variety of tissues and cell types. The other method commonly utilized in the isolation of phosphorylated acidic nuclear proteins is based on their solubility in phenol. The choice of assay conditions, the purity and physical state of the DNA, and the technique chosen to monitor protein–DNA binding are all important variables that must be considered when attempting to study the interaction of phosphorylated nonhistone proteins with DNA." @default.
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- W967059400 date "1975-01-01" @default.
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- W967059400 title "[14] Methods for analysis of phosphorylated acidic chromatin protein interactions with DNA" @default.
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- W967059400 doi "https://doi.org/10.1016/s0076-6879(75)40016-7" @default.
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