Matches in SemOpenAlex for { <https://semopenalex.org/work/W967595005> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W967595005 abstract "Publisher Summary This chapter describes the methods for the isolation of nuclei from ciliated protozoans. Structurally, macro- and micronuclei contain the same substructures as nuclei from other organisms, such as nuclear membranes, nucleoli, and chromatin elements. In some ciliates, giant polytene chromosomes develop in the anlagen; however, most macronuclei are organized into large and small bodies, which are considered to be nucleolarlike and chromatin bodies, respectively. The micronucleus usually has a more centrally located chromatin body, governs the germ line, divides mitotically, and is generally diploid. The chapter presents electron micrographs of a macronucleus and micronucleus isolated from Paramecium Aurelia . The ciliated protozoans can serve as model systems for the study of gene amplification, gene diminution, histone and nonhistone protein function, morphological development of chromosomes, and even as models of cellular aging. The chapter emphasizes the rationale necessary for the isolation of intact nuclei and the specific requirements regarding their use after isolation. Nuclei from ciliated protozoans can be isolated by a variety of methods. The chapter discusses the comparison between detergent and non-detergent methods. Both have their advantages with regard to cytoplasmic contamination, preservation of membrane, and in vitro enzyme activity." @default.
- W967595005 created "2016-06-24" @default.
- W967595005 creator A5034529485 @default.
- W967595005 date "1977-01-01" @default.
- W967595005 modified "2023-09-23" @default.
- W967595005 title "Chapter 5 Methods for the Isolation of Nuclei from Ciliated Protozoans" @default.
- W967595005 cites W1026181200 @default.
- W967595005 cites W1041627719 @default.
- W967595005 cites W1041813231 @default.
- W967595005 cites W1463780262 @default.
- W967595005 cites W1510567723 @default.
- W967595005 cites W1558826859 @default.
- W967595005 cites W1582603359 @default.
- W967595005 cites W1971290604 @default.
- W967595005 cites W1994025475 @default.
- W967595005 cites W2002018247 @default.
- W967595005 cites W2005063559 @default.
- W967595005 cites W2005436957 @default.
- W967595005 cites W2007664393 @default.
- W967595005 cites W2015390529 @default.
- W967595005 cites W2016478944 @default.
- W967595005 cites W2017156016 @default.
- W967595005 cites W2018491753 @default.
- W967595005 cites W2021481241 @default.
- W967595005 cites W2022298465 @default.
- W967595005 cites W2031679215 @default.
- W967595005 cites W2040360983 @default.
- W967595005 cites W2054620086 @default.
- W967595005 cites W2065482116 @default.
- W967595005 cites W2069600463 @default.
- W967595005 cites W2087011722 @default.
- W967595005 cites W2095575364 @default.
- W967595005 cites W2176878590 @default.
- W967595005 cites W2743041795 @default.
- W967595005 cites W643884882 @default.
- W967595005 doi "https://doi.org/10.1016/s0091-679x(08)60094-1" @default.
- W967595005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/407425" @default.
- W967595005 hasPublicationYear "1977" @default.
- W967595005 type Work @default.
- W967595005 sameAs 967595005 @default.
- W967595005 citedByCount "5" @default.
- W967595005 crossrefType "book-chapter" @default.
- W967595005 hasAuthorship W967595005A5034529485 @default.
- W967595005 hasConcept C104317684 @default.
- W967595005 hasConcept C143191323 @default.
- W967595005 hasConcept C178790620 @default.
- W967595005 hasConcept C183873130 @default.
- W967595005 hasConcept C185592680 @default.
- W967595005 hasConcept C190062978 @default.
- W967595005 hasConcept C2777334866 @default.
- W967595005 hasConcept C2777896290 @default.
- W967595005 hasConcept C2780536333 @default.
- W967595005 hasConcept C29730261 @default.
- W967595005 hasConcept C30481170 @default.
- W967595005 hasConcept C45080847 @default.
- W967595005 hasConcept C51423104 @default.
- W967595005 hasConcept C54355233 @default.
- W967595005 hasConcept C56683836 @default.
- W967595005 hasConcept C83640560 @default.
- W967595005 hasConcept C86803240 @default.
- W967595005 hasConcept C95444343 @default.
- W967595005 hasConceptScore W967595005C104317684 @default.
- W967595005 hasConceptScore W967595005C143191323 @default.
- W967595005 hasConceptScore W967595005C178790620 @default.
- W967595005 hasConceptScore W967595005C183873130 @default.
- W967595005 hasConceptScore W967595005C185592680 @default.
- W967595005 hasConceptScore W967595005C190062978 @default.
- W967595005 hasConceptScore W967595005C2777334866 @default.
- W967595005 hasConceptScore W967595005C2777896290 @default.
- W967595005 hasConceptScore W967595005C2780536333 @default.
- W967595005 hasConceptScore W967595005C29730261 @default.
- W967595005 hasConceptScore W967595005C30481170 @default.
- W967595005 hasConceptScore W967595005C45080847 @default.
- W967595005 hasConceptScore W967595005C51423104 @default.
- W967595005 hasConceptScore W967595005C54355233 @default.
- W967595005 hasConceptScore W967595005C56683836 @default.
- W967595005 hasConceptScore W967595005C83640560 @default.
- W967595005 hasConceptScore W967595005C86803240 @default.
- W967595005 hasConceptScore W967595005C95444343 @default.
- W967595005 hasLocation W9675950051 @default.
- W967595005 hasLocation W9675950052 @default.
- W967595005 hasOpenAccess W967595005 @default.
- W967595005 hasPrimaryLocation W9675950051 @default.
- W967595005 hasRelatedWork W1599834785 @default.
- W967595005 hasRelatedWork W1992907681 @default.
- W967595005 hasRelatedWork W2015474365 @default.
- W967595005 hasRelatedWork W2020103873 @default.
- W967595005 hasRelatedWork W2062799029 @default.
- W967595005 hasRelatedWork W2328999831 @default.
- W967595005 hasRelatedWork W2992231355 @default.
- W967595005 hasRelatedWork W3012986719 @default.
- W967595005 hasRelatedWork W3092652414 @default.
- W967595005 hasRelatedWork W5285446 @default.
- W967595005 isParatext "false" @default.
- W967595005 isRetracted "false" @default.
- W967595005 magId "967595005" @default.
- W967595005 workType "book-chapter" @default.