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- W968166321 abstract "经过硫酸铵分级沉淀、热处理、HiPrepTM Phenyl FF (high sub)疏水层析、DEAE Fastflow离子交换层析以及Sephadex G-200凝胶过滤,从Burkholderia cepecia.njut1中得到了海因酶,收率8.98%,纯化倍数为69.Burkholderia cepecia njut1海因酶是同源四聚体,亚基分子量52ku,等电点是5.09,具有严格的光学选择性,是D-型海因酶,并具有较广泛的底物适应性,对苄基海因和对羟苯海因有较高的亲和力.Burkholderia cepecia. njut1海因酶的最适催化的pH值为9.0. 酶在pH 6.5～10.0下稳定. 60℃时酶活力较高,但在此温度下酶的热稳定性较差.Burkholderia cepecia. njut1海因酶是金属依赖性酶,金属螯合剂EDTA对海因酶有抑制作用.二价金属离子对酶有较大的影响." @default.
- W968166321 created "2016-06-24" @default.
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- W968166321 date "2005-01-01" @default.
- W968166321 modified "2023-09-24" @default.
- W968166321 title "Burkholderia cepecia．njut1中的海因酶的纯化及性质" @default.
- W968166321 hasPublicationYear "2005" @default.
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