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- W968776768 abstract "This chapter describes an assay for fusion of intact viruses with biological targets that is based on the principle of fluorescence dequenching. According to the method, the fluorophore is incorporated into one of the fusing partners in such a way that its fluorescence is self-quenched. Once fusion has occurred, the fluorophore diffuses into the larger target, resulting in relief of self-quenching. Consequently, an increase in the fluorescence signal is observed. Fluorescence dequenching assays are developed for monitoring the mixing of lipids and aqueous spaces. Core-mixing assays for viral fusion require reconstitution of viral envelopes, whereas membrane mixing assays can be performed with intact virions. This chapter describes a lipid mixing assay which utilizes the lipophilic fluorescent dye octadecylrhodamine B (R 18). According to the R18 dequenching assay, fluorescence changes associated with membrane fusion in a virus–cell suspension are measured directly using a spectrofluorometer. This method requires relatively simple instrumentation and experimental conditions." @default.
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- W968776768 date "1993-01-01" @default.
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- W968776768 title "[21] Kinetics of fusion of enveloped viruses with cells" @default.
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- W968776768 doi "https://doi.org/10.1016/0076-6879(93)20089-l" @default.
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