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- W96889677 abstract "To develop a new SPE-HPLC method for the simultaneous determination of gallic acid, catechin and epicatechin in rhubarb decoction. The analytes were pretreated by solid-phase extraction using a C18 cartridge and separated on an Agilent Zorbax SB-C18 (250 mm × 4.6 mm, 5 m) analytical column by gradient elution using a mixture of methanol (0.06% formic acid) and water (0.1% formic acid) at 30 C. The method was found to be linear in the 4.66-233, 5.80-290 and 2.00-100 gmL−1 concentration range for gallic acid, catechin and epicatechin (r > 0.999), respectively. The overall RSD precision value (intra- and inter-day) for the retention times and peak-areas were less than 0.12% and 2.23%, respectively. In addition, the recoveries from spiked control samples were determined, ranging from 87.9% to 103%. The developed method was simple, specific and accurate, and can be applied for the quality control of the raw material of rhubarb and its corresponding remedies." @default.
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- W96889677 date "2012-07-01" @default.
- W96889677 modified "2023-10-16" @default.
- W96889677 title "A validated solid-phase extraction HPLC method for the simultaneous determination of gallic acid, catechin and epicatechin in rhubarb decoction" @default.
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- W96889677 doi "https://doi.org/10.1016/s1875-5364(12)60055-7" @default.
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