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- W970651158 abstract "Publisher Summary This chapter describes the method for the purification of plant mitochondria on silica sol gradients. Purification of washed mitochondria isolated by differential centrifugation from etiolated mung bean ( Phaseolus aureus ) hypocotyl and green pea ( Pisum sativum ) leaf tissues may be performed easily and rapidly using self-generated density gradients of the nontoxic, PVP-coated, silica sol Percoll. Mitochondria purified on Percoll gradients contain negligible levels of endogenous substrates, are devoid of galactolipids and carotenoids, and possess low levels of hydrolases such as lipolytic acyl hydrolases or lipoxygenase. They show markedly higher rates of O 2 consumption in state three than the unpurified mitochondria and have increased ADP/O and respiratory control ratios. Membrane intactness studies, as determined by KCN-sensitive ascorbate-cytochrome c-dependent O 2 consumption or succinate: cytochrome c-oxidoreductase, reveal that mitochondria purified in such a manner possess a mean percentage of intactness of approximately 95%. Thus, it is evident that the physiological integrity of mitochondria is maintained during the purification process." @default.
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- W970651158 date "1987-01-01" @default.
- W970651158 modified "2023-09-27" @default.
- W970651158 title "[38] Purification of plant mitochondria on silica sol gradients" @default.
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- W970651158 doi "https://doi.org/10.1016/0076-6879(87)48040-3" @default.
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