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- W970653806 abstract "Anliucheng (Citrus sinensis Osbeck), a very seedy and widely spread acidless sweet orange cultivar in south of China, was transformed by the strain of Agrobacterium Tumefaciens EHA105 carrying pTA29-barnase gene, which will induce pollen sterility in transgenic plants. The embryogenic calli of Anliucheng were co-cultivated with Agrobacterium tumefaciens for 3 days, and then transferred to selective medium containing 50 mg L-1 basta (a kind of herbicide) for 5 weeks. The resistant calli were recovered and regenerated 118 embryoids. A total of 13 entire plants were obtained after micro-grafted on trifoliate orange. These regenerated plants were verified by PCR amplification and confirmed by PCR-Southern blotting analysis." @default.
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- W970653806 date "2003-01-01" @default.
- W970653806 modified "2023-09-26" @default.
- W970653806 title "Agrobacterium—Mediated Transformation of Embryogenic Calli of Anliucheng and Regeneration of Plants Containing the Chimaeric Ribonuclease Gene" @default.
- W970653806 hasPublicationYear "2003" @default.
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