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- W970782965 abstract "This chapter focuses on the biology of the macrophage phagosome with respect to its changing responsibilities, although the data discussed is placed in context with other phagocytic cells. To determine the degradative capacity of the phagosome following its formation, fluorogenic substrates were coupled, along with a calibration fluor, to the surface of beads coated with the phagocytic receptor ligands immunoglobulin G (IgG) or mannosylated BSA. The acidification assay and the fluorescent resonance energy transfer (FRET) assay for phagosome/lysosome fusion both reach an end point equilibrium or maximum value. This contrasts with the hydrolysis assays that progress to substrate limitation because an enzyme, once present, will continue to hydrolyze its substrate until either the enzyme is removed or deactivated or, more likely, the substrate is exhausted. Some of these assays exploited specific substrates processed by single, identified enzymes, whereas other substrates were more generic and were hydrolyzed by several enzymes. Recently, these assays have been employed to probe the modulation of the macrophage phagosomes under differing biological situations such as microbial insult and cytokine-mediated activation. The chapter discusses immune activation and the physiology of the phagosome, and focuses specifically on the interplay between the two bacteria and the changing environments that they will experience in the phagosomes of the macrophage. Microarray analysis appears to afford novel insights into the intraphagosomal environment, with the important caveat that the physiology of these compartments is a reflection of both the host cell biology and its manipulation by the pathogen of interest." @default.
- W970782965 created "2016-06-24" @default.
- W970782965 creator A5003604608 @default.
- W970782965 creator A5013630399 @default.
- W970782965 creator A5030633948 @default.
- W970782965 creator A5068460948 @default.
- W970782965 date "2014-04-30" @default.
- W970782965 modified "2023-09-23" @default.
- W970782965 title "Functional Analysis of the Intraphagosomal Environment of the Macrophage: Fluorogenic Reporters and the Transcriptional Responses of Salmonella and Mycobacterium spp." @default.
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