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• W970838939 abstract "This chapter describes the methods for isolation of the mitotic apparatus (MA). The mitotic apparatus (spindle–aster–chromosome complex) functions during cell division to bring about an equal distribution of nuclear material to the daughter cells and is responsible for initiation of cytokinesis. The rationale for the DMSO–glycerol method is to isolate functional MA with stable microtubules. As the functional activity (chromosome movement) cannot be used to assess the merits of the MA isolation method, other criteria must be employed. These involve comparisons of optical and physicochemical properties of isolated MA with MA in vivo. Using a compound microscope with bright-field optics, the MA in vivo cannot be seen and isolates are barely visible. The MA in vivo are cold-labile, pressure-labile, and colchicine-sensitive. Depending upon the method of isolation, in vitro MA also displays lability to cold, hydrostatic pressure, and sensitivity to colchicine." @default.
• W970838939 created "2016-06-24" @default.
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• W970838939 date "1977-01-01" @default.
• W970838939 modified "2023-09-26" @default.
• W970838939 title "Chapter 23 The Mitotic Apparatus: Methods for Isolation" @default.
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• W970838939 doi "https://doi.org/10.1016/s0091-679x(08)60112-0" @default.
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• W970838939 hasPublicationYear "1977" @default.
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