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- W971128869 abstract "目的建立基因MLXIPL、PMVK、TRIB3启动子区DNA甲基化检测的方法。方法用亚硫酸氢钠和对苯二酚处理外周血细胞基因组DNA标本，以修饰后的DNA标本为模板，每个基因分别用内外侧两套不同的引物对启动子区进行巢式扩增。PCR产物进一步克隆、测序。结果测序结果表明，MLXIPL、PMVK、TRIB3启动子区基因中的非甲基化的C碱基转变为T碱基，而CpG岛被甲基化的C碱基则不改变。结论成功地建立了MLXIPL、PMVK、TRIB3基因甲基化检测的方法，为探索基因启动子区甲基化与疾病的关系做出了铺垫。" @default.
- W971128869 created "2016-06-24" @default.
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- W971128869 date "2010-01-01" @default.
- W971128869 modified "2023-09-24" @default.
- W971128869 title "MLXIPL、PMVK、TRIB3基因启动子区甲基化检测方法的建立" @default.
- W971128869 hasPublicationYear "2010" @default.
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