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- W971299457 abstract "Publisher Summary Cells from complex organisms contain DNA polymerase that may be unique in several details such as (1) the enzyme may require a denatured DNA template, (2) the enzyme may not form a firm association complex with DNA, and (3) the enzyme may be found in unexpected (extranuelear) cellular locations. The localization of DNA polymerase during the stages of the cell cycle may be of considerable importance in understanding the high degree of mitotic control in complex organisms. Detection of DNA polymerase requires the presence of deoxynucleoside triphosphate substrates and a suitable polydeoxynucleotide template. If these reagents could be introduced into the interior of a living cell, the presence of DNA polymerase might be detected. Introduction of the reagents could be performed by microinjection or by pinocytosis. The latter technique has been used with Amoeba proteus, because the result suggested the presence of DNA polymerase in Amoeba cytoplasm, the procedure is described for this case in the chapter. The results suggest the possibility that DNA polymerase may be a cytoplasmic enzyme during part of the cell cycle in Amoeba." @default.
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- W971299457 date "1968-01-01" @default.
- W971299457 modified "2023-09-27" @default.
- W971299457 title "[144] Demonstration of DNA polymerase in vivo" @default.
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