Matches in SemOpenAlex for { <https://semopenalex.org/work/W971918487> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W971918487 abstract "Background Complete congenital stationary night blindness (cCSNB) is a heterogeneous disorder of the retina characterized by impairment of low light vision and loss of the b-wave of the electroretinogram. Mutations in GRM6, TRPM1and NYX, which encode proteins critical to signal transmission in one class of retinal interneurons called depolarizing bipolar cells (DBCs) cause cCSNB. We have shown that an orphan G-protein coupled receptor, GPR179, also plays a vital role in DBC function. Methods/Results Immunochemical staining of retinal cross-sections demonstrates that TRPM1 and GPR179 co-localize on the dendritic tips of DBCs. Further, TRPM1 is lost from GPR179 mutant mice (Nob5 ) indicating they may interact. We used a yeast two-hybrid analyses and co-immunoprecipitation (co-IP) assays after expression in HEK293T cells to examine interactions. Both methods show that GPR179 and TRPM1 interact. Furthermore, antibodies to GPR179 could co-IP TRPM1 from retinal lysates, confirming the interaction. Conclusions This is the first study to demonstrate the interaction of TRPM1 and a previously uncharacterized orphan G protein receptor, GPR179. This result was confirmed by three independent methods suggesting that the interaction of GPR179 and TRPM1 is a critical component of the G protein signal transduction cascade in DBCs." @default.
- W971918487 created "2016-06-24" @default.
- W971918487 creator A5033322796 @default.
- W971918487 creator A5065223236 @default.
- W971918487 creator A5084345116 @default.
- W971918487 date "2012-04-01" @default.
- W971918487 modified "2023-09-24" @default.
- W971918487 title "GPR179 interacts with TRPM1 in retinal depolarizing bipolar cells" @default.
- W971918487 doi "https://doi.org/10.1096/fasebj.26.1_supplement.lb176" @default.
- W971918487 hasPublicationYear "2012" @default.
- W971918487 type Work @default.
- W971918487 sameAs 971918487 @default.
- W971918487 citedByCount "0" @default.
- W971918487 crossrefType "journal-article" @default.
- W971918487 hasAuthorship W971918487A5033322796 @default.
- W971918487 hasAuthorship W971918487A5065223236 @default.
- W971918487 hasAuthorship W971918487A5084345116 @default.
- W971918487 hasConcept C169760540 @default.
- W971918487 hasConcept C170493617 @default.
- W971918487 hasConcept C174510640 @default.
- W971918487 hasConcept C2777093970 @default.
- W971918487 hasConcept C2780827179 @default.
- W971918487 hasConcept C54355233 @default.
- W971918487 hasConcept C55493867 @default.
- W971918487 hasConcept C62478195 @default.
- W971918487 hasConcept C71829478 @default.
- W971918487 hasConcept C81885089 @default.
- W971918487 hasConcept C86803240 @default.
- W971918487 hasConcept C95444343 @default.
- W971918487 hasConceptScore W971918487C169760540 @default.
- W971918487 hasConceptScore W971918487C170493617 @default.
- W971918487 hasConceptScore W971918487C174510640 @default.
- W971918487 hasConceptScore W971918487C2777093970 @default.
- W971918487 hasConceptScore W971918487C2780827179 @default.
- W971918487 hasConceptScore W971918487C54355233 @default.
- W971918487 hasConceptScore W971918487C55493867 @default.
- W971918487 hasConceptScore W971918487C62478195 @default.
- W971918487 hasConceptScore W971918487C71829478 @default.
- W971918487 hasConceptScore W971918487C81885089 @default.
- W971918487 hasConceptScore W971918487C86803240 @default.
- W971918487 hasConceptScore W971918487C95444343 @default.
- W971918487 hasIssue "S1" @default.
- W971918487 hasLocation W9719184871 @default.
- W971918487 hasOpenAccess W971918487 @default.
- W971918487 hasPrimaryLocation W9719184871 @default.
- W971918487 hasRelatedWork W1480157153 @default.
- W971918487 hasRelatedWork W2039620761 @default.
- W971918487 hasRelatedWork W2082154568 @default.
- W971918487 hasRelatedWork W2083255178 @default.
- W971918487 hasRelatedWork W2085850146 @default.
- W971918487 hasRelatedWork W2138743300 @default.
- W971918487 hasRelatedWork W2138903088 @default.
- W971918487 hasRelatedWork W2140436003 @default.
- W971918487 hasRelatedWork W2188978850 @default.
- W971918487 hasRelatedWork W2343768960 @default.
- W971918487 hasVolume "26" @default.
- W971918487 isParatext "false" @default.
- W971918487 isRetracted "false" @default.
- W971918487 magId "971918487" @default.
- W971918487 workType "article" @default.