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- W97192057 abstract "ABSTRACT Mutation recH resulted in a poorly transformable and ATP-dependent DNase deficient mutant with higher sensitivity to UV and mitomycin C, while the frequency of transduction was not changed. Many spontaneous Rec+ revertants (mitomycin C-resistant) retaining low levels of ATP-dependent DNase were isolated and genetically assigned to three different map positions (transduction with phage AR9). Besides restoration of their transformability, no significant differences could be found between the recH mutant and its recombination proficient revertants when they were compared in different steps of genetic transformation process (binding and uptake of DNA, extent of its intracellular degradation, formation of both double-stranded and single-stranded donor-recipient DNA complexes and transforming activity of the donor DNA reextracted from the recipient cells). A strong indication of more than one recombination pathways in transformation resulted from these comparisons with no other conclusions concerning the role of the recH gene product." @default.
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- W97192057 date "1980-01-01" @default.
- W97192057 modified "2023-10-16" @default.
- W97192057 title "CHARACTERIZATION OF B. SUBTILIS recH MUTANT AND OF ITS REVERTANTS WITH LOW ACTIVITY OF ATP-DEPENDENT DNase" @default.
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- W97192057 doi "https://doi.org/10.1016/b978-0-08-025494-4.50047-4" @default.
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